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Miladinovic Sasa

Professeur-e HES Associé-e

Compétences principales

Professeur-e HES Associé-e

Bureau: ENP.19.N506

HES-SO Valais-Wallis - Haute Ecole d'Ingénierie
Rue de l'Industrie 23, 1950 Sion, CH

Domaine
Chimie et sciences de la vie

Filière principale
Ingénierie des Sciences du vivant

BSc HES-SO en Ingénierie des sciences du vivant - HES-SO Valais-Wallis - Haute Ecole d'Ingénierie

MSc HES-SO en Life Sciences - HES-SO Master

En cours

BioAI4LCMS: Automated LC-MS through AI- based and Generative Intelligence Technology

Rôle: Co-requérant(s)

Requérant(e)s: Panichella Sebastiano, Universität Bern

Description du projet :

BioAI4LCMS project will deliver a software platform that implements self-adaptive, AI-based mechanisms, designed to streamline and automate the development of methods for the Liquid chromatography-mass spectrometry process.

Equipe de recherche au sein de la HES-SO: Miladinovic Sasa

Partenaires académiques: Panichella Sebastiano, Universität Bern

Partenaires professionnels: Ossola Reto, Genedata AG

Statut: En cours

Terminés

Développement automatisé de souches bactériennes pour la bioproduction industrielle

AGP

Rôle: Collaborateur/trice

Requérant(e)s: VS - Institut Technologies du vivant

Financement: HES-SO Rectorat

Description du projet : Programme de recherche / Projets jeunes chercheurs 2023 Les objectifs généraux sont les suivants 1. Développer, optimiser et tester des souches bactériennes en utilisant les principes d'une biofonderie. Ces développements seront réalisés sur la base d'équipements existants et permettront d'établir les avantages et les défis de cette approche. 2. Evaluer et planifier la mise en place d'une nouvelle biofonderie dédiée, en partenariat avec des acteurs académiques et industriels régionaux.

Equipe de recherche au sein de la HES-SO: Wegmüller Sarah , Aeberli Luisa Fernanda , Kuhn Alexandre , Adler Aline , Miladinovic Sasa , Scotton Sarah , Marcon Nadia

Partenaires académiques: VS - Institut Technologies du vivant

Durée du projet: 01.01.2024 - 28.02.2025

Montant global du projet: 99'300 CHF

Statut: Terminé

2025

Quantitation of antibiotics in fresh fermentation medium by hydrophilic interaction chromatography mass spectrometry

Article scientifique ArODES

Nadia Marcon, Mathias Rüdt, Joachim Klein, Sasa Miladinovic

Analytical and bioanalytical chemistry, 2025, 417, 9, 1927-1934

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Résumé:

This study presents the development of a sophisticated liquid chromatography-mass spectrometry approach leveraging hydrophilic interaction chromatography (HILIC) for the quantification of kanamycin and spectinomycin in fermentation media. The method was validated per International Council for Harmonisation guidelines, demonstrating robust linearity, precision, and accuracy. To mitigate pronounced matrix effects common to complex fermentation matrices, sample preparation was thoroughly optimized with solid-phase extraction employing MCX sorbent, thereby enhancing recovery rates and minimizing analytical interference. The validated protocol demonstrated high correlation coefficients (R > 0.998), underscoring its robustness and reliability for the accurate quantification of antibiotics in challenging bioprocess environments, providing a valuable analytical tool for bioreactor system monitoring.

Quantitation of antibiotics in fresh fermentation medium by hydrophilic interaction chromatography mass spectrometry

Article scientifique

Marcon Nadia, Rüdt Matthias, Joachim Klein, Miladinovic Sasa

Analytical and Bioanalytical Chemistry, 2025 , pp. 1-8

Green analytical chemistry: integrating sustainability into undergraduate education

Article scientifique

Miladinovic Sasa

Analytical and Bioanalytical Chemistry, 2025

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2024

Localization of disulfide bonds in ribonuclease using low pH trypsin and LC-ESI-QTOF-MS

Article scientifique ArODES

Amélie Bornex, Sasa Miladinovic

CHIMIA, 2024, 78, 12, 881-882

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Résumé:

We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach. The goal was to minimize disulfide bond scrambling during sample preparation. By using N-ethylmaleimide (NEM) for alkylation of free cysteines, we achieved 92% sequence coverage and successfully identified the native disulfide bonds between specific cysteine residues. However, scrambled disulfide bonds were also observed. Our results indicate that while this low pH digestion method helps preserve native disulfide bonds, further refinement is needed to fully prevent disulfide bond rearrangement during sample preparation.

Green analytical chemistry :

Article scientifique ArODES

integrating sustainability into undergraduate education

Sasa Miladinovic

Analytical and Bioanalytical Chemistry, 2024, 417, 4, 665-673

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Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

Article scientifique

Amélie Bornex, Miladinovic Sasa

CHIMIA, 2024

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2023

Mycotoxin Recovery and Matrix Effect in Fava Bean Starch and Protein Isolate Measured by LC-MS/MS

Article scientifique

Miladinovic Sasa

CHIMIA, 2023

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2022

Chemical, Analytical and Pharmacokinetic Characterisation of RO7304898, an API Consisting of Two Rapidly Interconverting Diastereoisomers

Article scientifique

Katja Heinig, Filippo Sladojevich, Jeannine Schaffland, Georg Jaeschke, Alfred Ross, Philipp Koldewey, Miladinovic Sasa, Jin Wang, Caroline Rynn

Pharmaceutical Research, 2022

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Impact of charge patches on tumor disposition and biodistribution of therapeutic antibodies

Article scientifique

Jakob Stüber, Karin Rechberger, Miladinovic Sasa, Thomas Pöschinger, Tamara Zimmermann, Remi Villenave, Miro Eigenmann, Thomas Kraft, Dhaval Shah, Hubert Kettenberger, Wolfgang Richter

AAPS open, 2022

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2015

Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver microtissues*[s]

Article scientifique

Roland Bruderer, Oliver Bernhardt, Tejas Gandhi, Miladinovic Sasa, Lin-Yang Cheng, Simon Messner, Tobias Ehrenberger, Vito Zanotelli, Yulia Butscheid, Claudia Escher, Olga Vitek, Oliver Rinner, Lukas Reiter

Molecular & Cellular Proteomics, 2015

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2014

OpenSWATH enables automated, targeted analysis of data-independent acquisition MS data

Article scientifique

Hannes Röst, George Rosenberger, Pedro Navarro, Ludovic Gillet, Miladinovic Sasa, Olga Schubert, Witold Wolski, Ben Collins, Johan Malmström, Lars Malmström, Ruedi Aebersold

Nature biotechnology, 2014

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2012

In-spray supercharging of peptides and proteins in electrospray ionization mass spectrometry

Article scientifique

Miladinovic Sasa, Luca Fornelli, Yu Lu, Krzysztof Piech, Hubert Girault, Yury Tsybin

Analytical chemistry, 2012

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2008

Developments in MALDI Mass Spectrometry: The Quest for the Perfect Matrix

Article scientifique

Mariccor Batoy, Evgenia Akhmetova, Miladinovic Sasa, Joseph Smeal, Charles Wilkins