Description du projet :
Context of the project: Microbial polyesters, known as polyhydroxyalkanoates (PHA), are green materials that can have similar properties to petroleum-based plastics with a huge potential for biomedical applications. To optimize the bioprocess at its maximum, the establishment of routine and rapid analytical methods for assessing PHA production and degradation in laboratory and industrial scales are needed. Flow cytometry (FCM) is a powerful analytical tool, and the staining of cells with the appropriate dyes generate quantitative FCM results on the PHA production in just few minutes. The implementation of a robust at-line and automatic FCM analysis in the bioprocess would allow taking rapid responses, such as the adjustment of PHA precursor feeding and harvest of the cells at the optimal time point.
Content of the project:
The objective of the proposed project is to:
1) Implement a fluid handling system connected to a biorreactor allowing the autosampling of cultivation broth followed by dilution.
2) Development of an automatic FCM analysis of the sample collected and diluted by the fluid handling system. Feedback control of the fluid handling sytem (sample dilution) and the bioprocess (adjustment of PHA presursor feeding) using the data from FCM.
3) Validation of the automatized FCM system in bioprocesses for PHA production.
Benefit of the project:
In order to obtain funding from either private or public funding agencies for the further development of PAT bioprocess and for its evaluation in a technology transfer perspective, it is currently essential to have a working online analytics for the evaluation of the PHA content.
Justification of the required funding:
The cost related to the present project is mostly related to internal working hours. The work will mostly be shared between Aldo Vaccari, Camila Utsunomia and Manfred Zinn.
Research team within HES-SO:
Vaccari Aldo, Zinn Manfred, Utsunomia Camila
Durée du projet:
29.07.2019 - 31.12.2019
Montant global du projet: 19'642 CHF