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Simonin Scott

Simonin Scott

Adjoint scientifique

Main skills

Microbiology

Yeast

Œnology

Fermentation

Winemaking

Brewing

Distillation

  • Contact

  • Teaching

  • Publications

Main contract

Adjoint scientifique

Phone: +41 22 363 40 75

Changins- Haute école de viticulture et œnologie
Route de Duillier, 1260 Nyon, CH
EIC
BSc HES-SO en Oenologie - Changins- Haute école de viticulture et œnologie

2024

Applications of chitosan in the agri-food sector :
Scientific paper ArODES
a review

Mathilde Bertrand, Scott Simonin, Benoit Bach

Carbohydrate research,  2024, 543, 109219

Link to the publication

Summary:

Chitosan is a natural and renewable polysaccharide that can form biopolymers. It is derived from the deacetylation of chitin mainly from crustaceans' shells, but also from fungi and insects. Thanks to unique characteristics such as antimicrobial effects, antioxidant properties or film forming capacities, it has triggered an important amount of research in the last decade about possible applications in industrial fields. The main application field of chitosan is the food industry where it can be used for preservation purposes and shelf-life improvement for fresh food products such as fruits or meat. For beverages, it is used for clarification and fining as well as elimination of spoilage flora in beverages like fruit juices or wine. And in agriculture, it can be used as a plant protection product through different mechanisms like the elicitation of plant defences. The mechanisms of action of chitosan on microorganisms are multiple and complex but revolve mostly around the disturbance of microorganisms’ membranes and cell walls resulting in the leakage of cell material. The use of chitosan is still minor but is promising in finding environmentally friendly alternatives to synthetic chemicals and plastics. Therefore, its characterization is primordial for the future of sustainable production and preservation processes.

2023

Bioprospecting of a Metschnikowia pulcherrima indigenous strain for Chasselas winemaking in 2022 vintage
Scientific paper ArODES

Frederico Sizzano, Marie Blackford, Hélène Berthoud, Laurent Amiet, Sébastien Bailly, Frédéric Vuichard, Christine Monnard, Stefan Bieri, Jean-Laurent Spring, Yannick Barth, Corentin Descombes, François Lefort, Marilyn Cléroux, Scott Simonin, Charles Chappuis, Gilles Bourdin, Benoît Bach

Foods,  12, 24, 4485

Link to the publication

Summary:

Interest in Metschnikowia (M.) pulcherrima is growing in the world of winemaking. M. pulcherrima is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we describe the isolation, characterization, and use of an autochthonous strain of M. pulcherrima in the vinification of Chasselas musts from the 2022 vintage. M. pulcherrima was used in co-fermentation with Saccharomyces cerevisiae at both laboratory and experimental cellar scales. Our results showed that M. pulcherrima does not ferment sugars but has high metabolic activity, as detected by flow cytometry. Furthermore, sensory analysis showed that M. pulcherrima contributed slightly to the aromatic profile when compared to the control vinifications. The overall results suggest that our bioprospecting strategy can guide the selection of microorganisms that can be effectively used in the winemaking process.

Bio-protection in oenology by Metschnikowia pulcherrima :
Scientific paper ArODES
from field results to scientific inquiry

Maëlys Puyo, Scott Simonin, Benoît Bach, Géraldine Klein, Hervé Alexandre, Raphaëlle Tourdot-Maréchal

Frontiers in Microbiology,  2023, vol. 14, article no. 125973

Link to the publication

Summary:

Finding alternatives to the use of chemical inputs to preserve the sanitary and organoleptic quality of food and beverages is essential to meet public health requirements and consumer preferences. In oenology, numerous manufacturers already offer a diverse range of bio-protection yeasts to protect must against microbiological alterations and therefore limit or eliminate sulphites during winemaking. Bio-protection involves selecting non-Saccharomyces yeasts belonging to different genera and species to induce negative interactions with indigenous microorganisms, thereby limiting their development and their impact on the matrix. Although the effectiveness of bio-protection in the winemaking industry has been reported in numerous journals, the underlying mechanisms are not yet well understood. The aim of this review is to examine the current state of the art of field trials and laboratory studies that demonstrate the effects of using yeasts for bio-protection, as well as the interaction mechanisms that may be responsible for these effects. It focuses on the yeast Metschnikowia pulcherrima, particularly recommended for the bio-protection of grape musts.

Use of oenological tannins to protect the colour of rosé wine in a bioprotection strategy with metschnikowia pulcherrima
Scientific paper ArODES

Maëlys Puyo, Scott Simonin, Géraldine Klein, Vanessa David-Vaizant, Natalia Quijada-Morin, Hervé Alexandre, Raphaëlle Tourdot-Maréchal

Foods,  2023, vol. 12, no. 4, article no. 735

Link to the publication

Summary:

Although bioprotection is now recognised as an alternative to SO2 for limiting microbial spoilage, it does not guarantee protection against oxidation. This limits its application, more specifically for rosé winemaking. Oenological tannins present antioxidant properties, which could represent an interesting alternative to SO2 to protect must and wines against oxidation. A combination of the inoculation of a bioprotectant yeast strain and the addition of oenological tannins was tested to eliminate sulfites during the pre-fermentative step of rosé winemaking. In this experiment carried out in a winery, two oenological tannins were compared: quebracho and gall nut tannins. The antioxidant efficiency of tannins was compared to that of SO2. Colorimetric assays associated with chemical analyses of anthocyanins and phenolic compounds confirmed that the use of bioprotection alone did not protect the wine from oxidation. An addition of oenological tannins on musts stabilized the colour of bioprotected rosé wine in a similar way that SO2 addition did. Quebracho tannins appeared more efficient than gall nut tannins. The colour differences observed cannot be explained either by the concentration or forms of anthocyanins. However, the addition of tannins led to better protection of oxidation-sensitive phenolic compounds comparable to that obtained with the addition of sulfites.

Use of Oenological Tannins to Protect the Colour of Rosé Wine in a Bioprotection Strategy with Metschnikowia pulcherrima
Scientific paper

Maëlys Puyo, Simonin Scott, Géraldine Klein, Vanessa David-Vaizant, Natalia Quijada-Morin, Hervé Alexandre, Raphaëlle Tourdot-Maréchal

Foods, 2023 , vol.  12, no  (4)

Link to the publication

Summary:

Although bioprotection is now recognised as an alternative to SO2 for limiting microbial spoilage, it does not guarantee protection against oxidation. This limits its application, more specifically for rosé winemaking. Oenological tannins present antioxidant properties, which could represent an interesting alternative to SO2 to protect must and wines against oxidation. A combination of the inoculation of a bioprotectant yeast strain and the addition of oenological tannins was tested to eliminate sulfites during the pre-fermentative step of rosé winemaking. In this experiment carried out in a winery, two oenological tannins were compared: quebracho and gall nut tannins. The antioxidant efficiency of tannins was compared to that of SO2. Colorimetric assays associated with chemical analyses of anthocyanins and phenolic compounds confirmed that the use of bioprotection alone did not protect the wine from oxidation. An addition of oenological tannins on musts stabilized the colour of bioprotected rosé wine in a similar way that SO2 addition did. Quebracho tannins appeared more efficient than gall nut tannins. The colour differences observed cannot be explained either by the concentration or forms of anthocyanins. However, the addition of tannins led to better protection of oxidation-sensitive phenolic compounds comparable to that obtained with the addition of sulfites.

2022

Bioprotection on Chardonnay grape :
Scientific paper ArODES
limits and impacts of settling parameters

Scott Simonin, Carole Honoré-Chedozeau, Ludovic Monnin, Vanessa David-Vaizant, Benoît Bach, Hervé Alexandre, Bertrand Chatelet, Raphaëlle Tourdot-Marechal

Australian Journal of Grape and Wine Research,  2022, article no. 1489094

Link to the publication

Summary:

Background and Aims. If bioprotection has already been proven to limit the development of spoilage flora on musts, its effectiveness against oxidation depends on the winemaking process. To optimize its application, this study analyzed the evolution of the chemical composition of the bioprotected musts and wines, according to different settling routes. Their impacts on the organoleptic characteristics of wines were also studied. Methods and Results. A bioprotected must was subjected to 6 different maceration routes before AF (triplicates), varying the duration and temperature parameters. A temperature value ≤12°C was the main factor independently of the duration which allowed a good implantation of the bioprotectant. An increase of the maceration duration at 12°C led to browning of the must, without significant effect on the final color of the wine, which was felt as more “floral,” with more length in the mouth. Conclusions. The bioprotectant implantation and its effectiveness on the sensory profile of the wine was guaranteed at maceration temperature values lower than 12°C. Significance of the Study. This study participates in the improvement of the bioprotection management in white winemaking, with the guarantee of a positive impact of the prefermentation maceration without sulphites on the organoleptic profile of the wines.

2020

Bio-Protection as an Alternative to Sulphites: Impact on Chemical and Microbial Characteristics of Red Wines
Scientific paper

Simonin Scott, Chloé Roullier-Gall, Jordi Ballester, Philippe Schmitt-Kopplin, Beatriz Quintanilla-Casas, Stefania Vichi, Dominique Peyron, Hervé Alexandre, Raphaëlle Tourdot-Maréchal

Frontiers in Microbiology, 2020 , vol.  11

Link to the publication

Summary:

In wine, one method of limiting the addition of sulphites, a harmful and allergenic agent, is bio-protection. This practice consists of the early addition of microorganisms on grape must before fermentation. Non-Saccharomyces yeasts have been proposed as an interesting alternative to sulphite addition. However, scientific data proving the effectiveness of bio-protection remains sparse. This study provides the first analysis of the chemical and microbiological effects of a Metschnikowia pulcherrima strain inoculated at the beginning of the red winemaking process in three wineries as an alternative to sulphiting. Like sulphiting, bio-protection effectively limited the growth of spoilage microbiota and had no influence on the phenolic compounds protecting musts and wine from oxidation. The bio-protection had no effect on the volatile compounds and the sensory differences were dependent on the experimental sites. However, a non-targeted metabolomic analysis by FTICR-MS highlighted a bio-protection signature.

2018

Inoculation of Torulaspora delbrueckii as a bio-protection agent inwinemaking
Scientific paper

Simonin Scott, Hervé Alexandre, Maria Nikolantonaki, Christian Coelho, Raphaëlle Tourdot-Maréchal

Food Research International, 2018 , vol.  107, no  May 2018, pp.  451-461

Link to the publication

Summary:

In oenology, bio-protection consists in adding bacteria, yeasts or a mixture of microorganisms on grape must before fermentation in order to reduce the use of chemical compounds such as sulphites. More particularly, non-Saccharomyces yeasts are used as a total or partial alternative to sulphites. However, scientific data capable of proving the effectiveness of adding these yeasts on grape must is lacking. This study reports the analysis of antimicrobial and antioxidant effects of one non-Saccharomyces yeast, Torulaspora delbrueckii, inoculated at the beginning of the white winemaking process in two Burgundian wineries as an alternative to sulphiting. The implantation of the T. delbrueckii strain was successful in both wineries and had no impact on fermentation kinetics. Adding T. delbrueckii reduced biodiversity during the pre-fermentation stages compared to sulphited controls and it also effectively limited the development of spoilage microorganisms in the same way as the addition of sulphites. T. delbrueckii could protect must and wine from oxidation as demonstrated by the analysis of colour and phenolic compounds. This is the first evidence that early addition of T. delbrueckii during wine-making can be a microbiogical and chemical alternative to sulphites. However, its contribution seems to bematrix dependent.
 

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