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Jovic Milica

Collaborateur-trice scientifique HES

Main skills

Point-of-care diagnostics (POCT)

Collaborateur-trice scientifique HES

Phone: +41 58 606 88 75

Desktop: ENP.19.N108c

HES-SO Valais-Wallis - Haute Ecole d'Ingénierie
Rue de l'Industrie 23, 1950 Sion, CH

MSc HES-SO en Life Sciences - HES-SO Master

projets de Master

Completed

Axe Santé 2022 - HES-SO Valais-Wallis

AGP

Role: Collaborator

Financement: VS - Direction / Ra&D

Description du projet : Projet inter-disciplinaire pour l'Axe Santé de la HES-SO Valais-Wallis Principalement saisie d'heures pour la coordination et la participation aux séances de l'axe. Pas de remboursement de frais de déplacement inter-site. Ventilation des BSM : W00 - CC90014 - n° projet (ne pas mettre de pilier 6 dans la ventilation car c'est sur un centre de coûts) Répartition du budget sur les projets socle des instituts au 31.12.2022

, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , Research team within HES-SO: Fragniere Emmanuel Prim Denis Bonazzi Riccardo Mathieu Marc Gallay Steve Zinn Manfred Loubier Jean-Christophe Antic Dragana Lugon Ralph Gaillard Vanessa Loup Jimmy Martinovic Jelena Barbey Valérie Geiser Martial Fournier Claude-Alexandre Schumacher Michael Ignaz Pichonnaz David Pralong Jean Gafner Simone Nanchen Benjamin Müller Henning Hilfiker Roger Solioz Emmanuel Calbimonte Jean-Paul Darbellay Anne Schnyder Bruno Simalatsar Alena Calvaresi Davide Tagan David Segura Jean-Manuel Bassolino Michela Dini Sarah Rey-Mermet Samuel Jovic Milica Cinna Adeline Jerjen Livia Delaloye Matthieu de Preux-Allet Lara Pfeifer Marc Emil Cotting Alexandre Pignat Marc

Partenaires académiques: VS - Institut Technologies du vivant

Durée du projet: 01.01.2022 - 31.12.2022

Montant global du projet: 200'000 CHF

Statut: Completed

Axe Santé 2020 - HES-SO Valais-Wallis

AGP

Role: Collaborator

Financement: VS - Direction / Ra&D; VS - Direction / Ra&D

Research team within HES-SO: Prim Denis , Depeursinge Adrien , Bonazzi Riccardo , Loubier Jean-Christophe , Lugon Ralph , Weissbrodt Rafael , Verloo Henk , Antille Benoît , Zappellaz Nancy , Geiser Martial , Fournier Claude-Alexandre , Schumacher Michael Ignaz , Carrard Sophie , Bruchez Lise , Eggenschwiler Stefan , Caliesch Rahel , Soutrenon Mathieu , Aissaoui Djamel , Müller Henning , Hilfiker Roger , Solioz Emmanuel , Manzo Gaetano , Calbimonte Jean-Paul , Sattelmayer Martin , Simalatsar Alena , Giacomino Katia , Calvaresi Davide , Andrearczyk Vincent , Kaufmann Anne-Laure , Mastelic Joëlle , Segura Jean-Manuel , Bassolino Michela , Dini Sarah , Jovic Milica , Antille Alain , Dreyer-Cornut Sonam , Delaloye Matthieu , de Preux-Allet Lara , Voll Peter , Pfeifer Marc Emil , Cotting Alexandre , Eggel Ivan

Durée du projet: 01.01.2020 - 31.12.2020

Montant global du projet: 250'000 CHF

Statut: Completed

2023

Biomarker-based diagnostics for mild traumatic brain injury (mTBI) at POC - rising to the challenges

Professional paper ArODES

Marc E. Pfeifer, Milica Jovic, Denis Prim

CHIMIA, 77, 7/8, 536

Link to the publication

A novel point-of-care diagnostic prototype system for the simultaneous electrochemiluminescent sensing of multiple traumatic brain injury biomarkers

Scientific paper ArODES

Milica Jovic, Denis Prim, Ophélie Righini, David Tagan, Mélanie Stäuble, Marc Pignat, Steve Gallay, Martial Geiser, Marc E. Pfeifer

Sensors & Diagnostics, 2, 4, 964-975

Link to the publication

Summary:

Traumatic brain injuries (TBI) are typically acquired when a sudden violent event causes damage to the brain tissue. A high percentage (70–85%) of all TBI patients are suffering from mild TBI (mTBI), which is often difficult to detect and diagnose with standard imaging tools (MRI, CT scan) due to the absence of significant lesions and specific symptoms. Recent studies suggest that a screening test based on the measurement of a protein biomarker panel directly from a patient's blood can facilitate mTBI diagnosis. Herein, we report a novel prototype system designed as a precursor of a future hand-held point-of-care (POC) diagnostic device for the simultaneous multi-biomarker sensing, employing a microarray-type spatially resolved electrochemiluminescence immunoassay (SR-ECLIA). The small tabletop prototype consists of a screen-printed electrode compartment to conduct multi-analyte ECL sandwich assays, a potentiostat module and a light collection module, all integrated into a compact 3D-printed housing (18.2 × 16.5 × 5.0 cm), as well as an sCMOS detector. Based on this design concept, further miniaturization, system integration, performance optimization and clinical evaluation shall pave the way towards the development of a portable instrument for use at the site of accident and healthcare. To demonstrate the system's feasibility, current performance and efficiency, the simultaneous detection of three mTBI biomarkers (GFAP, h-FABP, S100β) in 50% serum was achieved in the upper pg mL−1 range. The proposed device is amenable to the detection of other biomarker panels and thus could open new medical diagnostic avenues for sensitive multi-analyte measurements with low-volume biological sample requirements

Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry

Book chapter

Prim Denis, Jovic Milica, Pfeifer Marc Emil

Dans Marina Cretich, AlessandroGori, Peptide Microarrays. 2023, New York U.S.A : Humana press

Link to the publication

Opening the black box of traumatic brain injury: a holistic approach combining human 3D neural tissue and an in vitro traumatic brain injury induction device

Scientific paper

Céline Loussert-Fonta, Stoppini Luc, Yoan Neuenschwander, Ophélie Righini, Prim Denis, Cédric Schmidt, Heuschkel Marc, Loris Gomez Baisac, Jovic Milica, Pfeifer Marc Emil, Extermann Jérôme, Roux Adrien

Frontiers in Neuroscience, 2023 , vol. 17, no 1189615

Link to the publication

2022

Towards a Point-of-Care (POC) Diagnostic Platform for the Multiplex Electrochemiluminescent (ECL) Sensing of Mild Traumatic Brain Injury (mTBI) Biomarkers

Scientific paper

Jovic Milica, Prim Denis, Edis Saini, Pfeifer Marc Emil

Biosensors, 2022 , vol. 12, no 172

Link to the publication

2021

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics :

Scientific paper ArODES

towards a point-of-care application

Nadezda Pankratova, Milica Jovic, Marc E. Pfeifer

RSC Advances, 2021, vol. 11, pp. 17301-17319

Link to the publication

Summary:

Traumatic Brain Injury (TBI) being one of the principal causes of death and acquired disability in the world imposes a large burden on the global economy. Mild TBI (mTBI) is particularly challenging to assess due to the frequent lack of well-pronounced post-injury symptoms. However, if left untreated mTBI (especially when repetitive) can lead to serious long-term implications such as cognitive and neuropathological disorders. Computer tomography and magnetic resonance imaging commonly used for TBI diagnostics require well-trained personnel, are costly, difficult to adapt for on-site measurements and are not always reliable in identifying small brain lesions. Thus, there is an increasing demand for sensitive point-of-care (POC) testing tools in order to aid mTBI diagnostics and prediction of long-term effects. Biomarker quantification in body fluids is a promising basis for POC measurements, even though establishing a clinically relevant mTBI biomarker panel remains a challenge. Actually, a minimally invasive, rapid and reliable multianalyte detection device would allow the efficient determination of injury biomarker release kinetics and thus support the preclinical evaluation and clinical validation of a proposed biomarker panel for future decentralized in vitro diagnostics. In this respect electrochemical biosensors have recently attracted great attention and the present article provides a critical study on the electrochemical protocols suggested in the literature for detection of mTBI-relevant protein biomarkers. The authors give an overview of the analytical approaches for transduction element functionalization, review recent technological advances and highlight the key challenges remaining in view of an eventual integration of the proposed concepts into POC diagnostic solutions.

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

Scientific paper

Nadezda Pankratova, Jovic Milica, Pfeifer Marc Emil

RSC Advances, 2021 , vol. 11

Link to the publication

Achievements

PEOPLE@HES-SO
Directory and Skills inventory

Jovic Milica

Collaborateur-trice scientifique HES

Main skills

Bioanalytique

Immunoassays

Point-of-care diagnostics (POCT)

Electrochemistry

In vitro Diagnostics

Microfabrication

Sensors

Collaborateur-trice scientifique HES

PEOPLE@HES-SO Directory and Skills inventory

Jovic Milica

Collaborateur-trice scientifique HES

Main skills

Bioanalytique

Immunoassays

Point-of-care diagnostics (POCT)

Electrochemistry

In vitro Diagnostics

Microfabrication

Sensors

Collaborateur-trice scientifique HES

PEOPLE@HES-SO
Directory and Skills inventory