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Charmet Jérôme

Professeur-e HEs associé-e B

Main skills

Professeur-e HEs associé-e B

Haute Ecole Arc - Ingénierie
Espace de l'Europe 11, 2000 Neuchâtel, CH

BSc HES-SO en Microtechniques - Haute Ecole Arc - Ingénierie

Chimie générale
Biophysique
Biomatériaux et biocompatibilité
Technologie médicales

Ongoing

DNAMIC

Role: Co-applicant

Description du projet :

EIC pathfinder - starting officially 1st October 2023

come back soon for updates

Research team within HES-SO: Charmet Jérôme

Durée du projet: 01.10.2023 - 30.09.2026

Montant global du projet: 4'951'890 CHF

Statut: Ongoing

Misc projets

Role: Main Applicant

Description du projet :

Projets de recherche en tant que Professeur Associé à l'Université de Warwick.

(total > £2 mio. entre 2016-2021 en tant qu’investigateur principal)

• Feeling the force: Enhancing OOAC capability for biomechanical assessment and application. UK Research and Innovation (Co-investigateur, £23k), mars 2021.
• Organ-on-chip platform for TEER imaging, UK Research and Innovation (£30k), janvier 2021.
• Capacity Building for Prototyping Compatible Injection Moulding, High Value Manufacturing (£236k, trois partenaires industriels), mars 2020.
• Identification of metastatic biomarkers in nasopharyngeal carcinoma (NPC) to improve clinical management, Global Challenges Research Fund, (Co-investigateur, £50k), décembre 2019.
• MICRA Commercialisation Development Grant Award (£19k, deux partenaires industriels), dé-cembre 2019.
• Engineering Cardiovascular Systems using Protein Scaffolds, Monash-Warwick Alliance, Acceler-ator project (£247k), juillet 2019.
• Blood-brain-barrier on a chip to study the clinical relevance of circadian timing of anticancer drugs. Royal Society RGS\R2\180434 (£19k), mars 2019.
• Microfabrication suite. Capital Award for ECR. Engineering and Physical Science Research Coun-cil (EPSRC) EP/S017887/1 (£80k), janvier 2019.
• PleuSense, Differential diagnosis of pleural effusion, Institutional Links grant, ID 352360246, under the Newton-Katip Celebi Fund partnership, (£288k, deux partenaires industriels), février 2018.
• mPatch, mobile device for fitness and health monitoring. High Value Manufacturing Catapult (£213k, deux partenaires industriels), octobre 2017.
• Integrated platform with MEMS cantilever for the early detection of prostate cancer using dry mass sensing. EPSRC EP/R00403X/1 (£126k, un partenaire industriel), octobre 2017.
• Diamond Light Source beamtime, Beamline B22, B23 (67 shifts, ~£580k, deux partenaires indus-triels), depuis mars 2017.
• Mandats Industriels (£205k), depuis janvier 2017.
• University of Warwick; Research development fund, Global Fund partnership, Global Research Priority (total: £40k), depuis mars 2017.

Research team within HES-SO: Charmet Jérôme

Durée du projet: - 14.02.2022

Montant global du projet: 2'500'000 CHF

Statut: Ongoing

Public Mask

Role: Co-applicant

Financement: HES-SO

Description du projet :

Développement du filtre d’un masque de protection jetable destiné au grand public en appliquant l’electrospinning d’un polymère biodégradable.

Research team within HES-SO: Charmet Jérôme , Hengsberger Stefan , Marti Roger

Statut: Ongoing

2023

Effects of processing-induced contamination on organic electronic devices

Scientific paper ArODES

Dimitrios Simatos, Ian E. Jacobs, Illia Dobryden, Malgorzata Nguyen, Achilleas Savva, Deepak Venkateshvaran, Mark Nikolka, Jérôme Charmet, Leszek J. Spalek, Mindaugus Gicevicius, Youcheng Zhang, Guillaume Schweicher, Duncan J. Howe, Sarah Ursel, John Armitage, Ian B. Dimov, Ulrike Kraft, Weimin Zhang, Maryam Alsufyani, Iain McCulloch, M. Roisin Owens, Per M. Claesson, Tuomas P. J. Knowles, Henning Sirringhaus

Small Methods,

Link to the publication

Summary:

Organic semiconductors are a family of pi-conjugated compounds used in many applications, such as displays, bioelectronics, and thermoelectrics. However, their susceptibility to processing-induced contamination is not well understood. Here, it is shown that many organic electronic devices reported so far may have been unintentionally contaminated, thus affecting their performance, water uptake, and thin film properties. Nuclear magnetic resonance spectroscopy is used to detect and quantify contaminants originating from the glovebox atmosphere and common laboratory consumables used during device fabrication. Importantly, this in-depth understanding of the sources of contamination allows the establishment of clean fabrication protocols, and the fabrication of organic field effect transistors (OFETs) with improved performance and stability. This study highlights the role of unintentional contaminants in organic electronic devices, and demonstrates that certain stringent processing conditions need to be met to avoid scientific misinterpretation, ensure device reproducibility, and facilitate performance stability. The experimental procedures and conditions used herein are typical of those used by many groups in the field of solution-processed organic semiconductors. Therefore, the insights gained into the effects of contamination are likely to be broadly applicable to studies, not just of OFETs, but also of other devices based on these materials.

Platform-agnostic electrochemical sensing app and companion potentiostat

Scientific paper ArODES

Vijayalaxmi Manoharan, Rui Rodrigues, Sara Sadati, Marcus B. Swann, Neville Freeman, Bowen Du, Ender Yildirim, Ugur Tamer, Theodoros N. Arvanitis, Dmitry Isakov, Ali Asadipour, Jérôme Charmet

The Analyst,

Link to the publication

Summary:

Electrochemical sensing is ubiquitous in a number of fields ranging from biosensing, to environmental monitoring through to food safety and battery or corrosion characterisation. Whereas conventional potentiostats are ideal to develop assays in laboratory settings, they are in general, not well-suited for field work due to their size and power requirements. To address this need, a number of portable battery-operated potentiostats have been proposed over the years. However, most open source solutions do not take full advantage of integrated circuit (IC) potentiostats, a rapidly evolving field. This is partly due to the constraining requirements inherent to the development of dedicated interfaces, such as apps, to address and control a set of common electrochemical sensing parameters. Here we propose the PocketEC, a universal app that has all the functionalities to interface with potentiostat ICs through a user defined property file. The versatility of PocketEC, developed with an assay developer mindset, was demonstrated by interfacing it, via Bluetooth, to the ADuCM355 evaluation board, the open-source DStat potentiostat and the Voyager board, a custom-built, small footprint potentiostat based around the LMP91000 chip. The Voyager board is presented here for the first time. Data obtained using a standard redox probe, Ferrocene Carboxylic Acid (FCA) and a silver ion assay using anodic stripping multi-step amperometry were in good agreement with analogous measurements using a bench top potentiostat. Combined with its Voyager board companion, the PocketEC app can be used directly for a number of wearable or portable electrochemical sensing applications. Importantly, the versatility of the app makes it a candidate of choice for the development of future portable potentiostats. Finally, the app is available to download on the Google Play store and the source codes and design files for the PocketEC app and the Voyager board are shared via Creative Commons license (CC BY-NC 3.0) to promote the development of novel portable or wearable applications based on electrochemical sensing.

X-ray computed tomography for predictive quality assessment, 3D visualisation of micro-injection mouldings and soft-tool deformation

Scientific paper ArODES

Mert Gülçür, Paul Wilson, Michael Donnelly, Kevin Couling, Vanessa Goodship, Jérôme Charmet, Mark A. Williams, Gregory Gibbons

Materials Design, 2023, vol. 227, article no. 111741

Link to the publication

Summary:

This work presents X-ray computed tomography (XCT) as a dimensional quality assurance technique for micro-injection moulded polymeric test objects for the establishment of predictive quality models and quantifying soft-tool deformation. The results are compared against an industry standard laser-scanning-confocal microscope (LSCM) for the evaluation of XCT’s capability. The work demonstrates; (i) the exploitation of a XCT equipment for dimensional characterisation of micro-injection moulded products made out of polymers with adequate acquisition times, (ii) that acquired XCT data from the 3D visualisation of the micromouldings perform on par with a laser-scanning-confocal microscope in a quality prediction model, (iii) that the deformation occurring in an additively manufactured soft-tool can be quantified using XCT. The technique was particularly superior in volumetric data acquisition compared to LSCM in the filling prediction of the micromouldings. Better accuracy and repeatability in predicting the quality of the mouldings up to 92% achieved with XCT, in conjunction with an in-line collected soft-tool surface temperature data as an indirect quality assurance method. Given the capability of the XCT for the 3D data acquisition of polymeric miniature components, the approach described here has great potential in high-value micro-manufacturing process quality modelling for in-line quality assessment of miniature and added value products in data-rich contexts. Rendered 3D animation of the X-ray CT data: https://youtu.be/KwZty_yoDfs.

Rapid tooling :

Scientific paper ArODES

investigation of soft-tooled micro-injection moulding process characteristics using in-line measurements and surface metrology

Mert Gülçür, Kevin Couling, Vannessa Goodship, Jérôme Charmet, Gregory J. Gibbons

Rapid Prototyping Journal, To be published.

Link to the publication

Summary:

The purpose of this study is to demonstrate and characterise a soft-tooled micro-injection moulding process through in-line measurements and surface metrology using a data-intensive approach. A soft tool for a demonstrator product that mimics the main features of miniature components in medical devices and microsystem components has been designed and fabricated using material jetting technique. The soft tool was then integrated into a mould assembly on the micro-injection moulding machine, and mouldings were made. Sensor and data acquisition devices including thermal imaging and injection pressure sensing have been set up to collect data for each of the prototypes. Off-line dimensional characterisation of the parts and the soft tool have also been carried out to quantify the prototype quality and dimensional changes on the soft tool after the manufacturing cycles. The data collection and analysis methods presented here enable the evaluation of the quality of the moulded parts in real-time from in-line measurements. Importantly, it is demonstrated that soft-tool surface temperature difference values can be used as reliable indicators for moulding quality. Reduction in the total volume of the soft-tool moulding cavity was detected and quantified up to 100 cycles. Data collected from in-line monitoring was also used for filling assessment of the soft-tool moulding cavity, providing about 90% accuracy in filling prediction with relatively modest sensors and monitoring technologies. This work presents a data-intensive approach for the characterisation of soft-tooled micro-injection moulding processes for the first time. The overall results of this study show that the product-focussed data-rich approach presented here proved to be an essential and useful way of exploiting additive manufacturing technologies for soft-tooled rapid prototyping and new product introduction.

Flow rate-independent multiscale liquid biopsy for precision oncology

Scientific paper ArODES

Jie Wang, Robert Dallmann, Renquan Lu, Jing Yan, Jérôme Charmet

ACS Sensors, To be published.

Link to the publication

Summary:

Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management but typically suffer from low throughput, relative complexity, and postprocessing limitations. Here, we address these issues simultaneously by decoupling and independently optimizing the nano-, micro-, and macro-scales of an enrichment device that is simple to fabricate and operate. Unlike other affinity-based devices, our scalable mesh approach enables optimum capture conditions at any flow rate, as demonstrated with constant capture efficiencies, above 75% between 50 and 200 μL min–1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. We demonstrate its postprocessing capacity with the identification of potential responders to immune checkpoint inhibition (ICI) therapy and the detection of HER2 positive breast cancer. The results compare well with other assays, including clinical standards. This suggests that our approach, which overcomes major limitations associated with affinity-based liquid biopsies, could help improve cancer management.

2022

Multifunctional composite hydrogels for bacterial capture, growth/elimination, and sensing applications

Scientific paper ArODES

Andrea Dsouza, Chrystala Constatinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet, Rachel A. Hand

ACS Applied Materials Interfaces, To be published

Link to the publication

Summary:

Hydrogels are crosslinked networks of hydrophilic polymer chains with a three-dimensional structure. Owing to their unique features, the application of hydrogels for bacterial/antibacterial studies and bacterial infection management has grown in importance in recent years. This trend is likely to continue due to the rise in bacterial infections and antimicrobial resistance. By exploiting their physiochemical characteristics and inherent nature, hydrogels have been developed to achieve bacterial capture and detection, bacterial growth or elimination, antibiotic delivery, or bacterial sensing. Traditionally, the development of hydrogels for bacterial/antibacterial studies have focused on achieving a single function such as antibiotic delivery, antibacterial activity, bacterial growth, or bacterial detection. However, recent studies demonstrate the fabrication of hydrogels that exhibit multifunctionality in the form of multifunctional hydrogels, where a single hydrogel is capable of performing more than one bacterial/antibacterial function or composite hydrogels consisting of a number of single functionalized hydrogels, which exhibit bacterial/antibacterial function synergistically. In this review, we first highlight the hydrogel features critical for bacterial studies and infection management. Then, we specifically address unique hydrogel properties, their surface/network functionalization, and mode of action for bacterial capture, adhesion/growth, antibacterial activity, and bacterial sensing respectively. Finally, we provide insights into different strategies for developing multifunctional hydrogels and how such systems can help tackle, manage, and understand bacterial infections and antimicrobial resistance. We also note that the strategies highlighted in this review can be adapted to other cell types and are therefore likely to find applications beyond the field of microbiology.

Flow rate independent multiscale liquid biopsy for precision oncology

Scientific paper ArODES

Jing Yan, Jie Wang, Robert Dallmann, Renquan Lu, Jérôme Charmet

arXiv.org,

Link to the publication

Summary:

Immunoaffinity-based liquid biopsies of circulating tumour cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues simultaneously by decoupling and independently optimising the nano-, micro- and macro-scales of a CTC enrichment device that is both simple to fabricate and operate. At its core is a scalable macroscale mesh with optimised micropores, nanofunctionalised with antibodies against cell surface proteins. Unlike other affinity-based liquid biopsies, optimum capture can be achieved independently of the flow rate, as demonstrated with constant capture efficiencies, above 75%, between 50–200 µL min-1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. To demonstrate its post-processing capabilities, we used immunofluorescence labelling to identify PD-L1+ CTCs in 36% of patients (n=33) as potential responders to immune checkpoint inhibition therapy. Finally, our device achieved an 80% positive match in the identification of HER2+ breast cancer (n=26) compared to clinical standard FISH on solid biopsy. The results suggest that our approach, which overcomes major limitations previously associated with affinity-based liquid biopsies, could provide a versatile tool to improve cancer management.

P10 rapid capture of uropathogenic bacteria and on-chip determination of antimicrobial resistance

Professional paper ArODES

Andrea Dsouza, Rachel Hand, Chrystala Constantinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet

JAC-Antimicrobial Resistance, 2022, vol. 4, suppl. no. 1

Link to the publication

Publications (sélection parmi >50 publications, >1200 citations, h-index 18)

Scientific paper

Charmet Jérôme

Selection, 2022

Summary:

Liste complète ici

T. Kartanas et al., Analytical Chemistry 93, 2848, 2021
T. Kartanas et al., Applied Physics Letters, 116, 153702, 2020.
J. Charmet et al., Micromachines, 11, 135, 2020.
C. Bortolini, et al. Lab on a Chip, 19, 50, 2019.
R.S. Ruggeri, et al. Nature Communication 9, 3890, 2018
W.E. Arter, et al. Analytical Chemistry. 90, 10302, 2018.
J. Charmet, P. Arosio, T. P. J. Knowles, Journal of Molecular Biology. 430, 565, 2018.
T. Kartanas et al., Analytical Chemistry. 89, 11929, 2017.
M. Nikolka, et al., Nature Materials. 16, 356, 2017.
P. K. Challa, et al., Biomicrofluidics 11, 0141131, 2017.
J. Charmet, et al., Physical Review Applied. 5, 064016, 2016.
A. Prasad, J. Charmet, A. A. Seshia, Sensors and Actuators A Physical. 238, 207, 2016.
J. Charmet, et al., Applied Physics Letters. 105, 013502, 2014.

2022

Flow rate effect on THz-TDS of thin-film fluid in microfluidic device

Conference ArODES

Xuefei Ding, Jérôme Charmet, Emma Pickwell-McPherson

Proceedings of the 2022 47th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz), 28 August - 2 September 2022, Delft, Netherlands

Link to the conference

Summary:

Here we present a compact microfluidic device for thin-film water fluid measurements in reflection THz-TDS. We discuss the variables that need to be considered when utilizing this device in THz-TDS, and we find that different flow rates can cause subtle changes in the THz response.

Achievements

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Charmet Jérôme

Professeur-e HEs associé-e B

Main skills

Microfabrication

Biosensors

Microfluidics

Polymer technology

Functional materials

Professeur-e HEs associé-e B