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Charmet Jérôme

Professeur-e HEs associé-e B

Main skills

Professeur-e HEs associé-e B

Haute Ecole Arc - Ingénierie
Espace de l'Europe 11, 2000 Neuchâtel, CH

BSc HES-SO en Microtechniques - Haute Ecole Arc - Ingénierie

Chimie générale
Biophysique
Biomatériaux et biocompatibilité
Technologie médicales

Ongoing

Misc projets

Role: Main Applicant

Description du projet :

Projets de recherche en tant que Professeur Associé à l'Université de Warwick.

(total > £2 mio. entre 2016-2021 en tant qu’investigateur principal)

• Feeling the force: Enhancing OOAC capability for biomechanical assessment and application. UK Research and Innovation (Co-investigateur, £23k), mars 2021.
• Organ-on-chip platform for TEER imaging, UK Research and Innovation (£30k), janvier 2021.
• Capacity Building for Prototyping Compatible Injection Moulding, High Value Manufacturing (£236k, trois partenaires industriels), mars 2020.
• Identification of metastatic biomarkers in nasopharyngeal carcinoma (NPC) to improve clinical management, Global Challenges Research Fund, (Co-investigateur, £50k), décembre 2019.
• MICRA Commercialisation Development Grant Award (£19k, deux partenaires industriels), dé-cembre 2019.
• Engineering Cardiovascular Systems using Protein Scaffolds, Monash-Warwick Alliance, Acceler-ator project (£247k), juillet 2019.
• Blood-brain-barrier on a chip to study the clinical relevance of circadian timing of anticancer drugs. Royal Society RGS\R2\180434 (£19k), mars 2019.
• Microfabrication suite. Capital Award for ECR. Engineering and Physical Science Research Coun-cil (EPSRC) EP/S017887/1 (£80k), janvier 2019.
• PleuSense, Differential diagnosis of pleural effusion, Institutional Links grant, ID 352360246, under the Newton-Katip Celebi Fund partnership, (£288k, deux partenaires industriels), février 2018.
• mPatch, mobile device for fitness and health monitoring. High Value Manufacturing Catapult (£213k, deux partenaires industriels), octobre 2017.
• Integrated platform with MEMS cantilever for the early detection of prostate cancer using dry mass sensing. EPSRC EP/R00403X/1 (£126k, un partenaire industriel), octobre 2017.
• Diamond Light Source beamtime, Beamline B22, B23 (67 shifts, ~£580k, deux partenaires indus-triels), depuis mars 2017.
• Mandats Industriels (£205k), depuis janvier 2017.
• University of Warwick; Research development fund, Global Fund partnership, Global Research Priority (total: £40k), depuis mars 2017.

Research team within HES-SO: Charmet Jérôme

Durée du projet: - 14.02.2022

Montant global du projet: 2'500'000 CHF

Statut: Ongoing

Public Mask

Role: Co-applicant

Financement: HES-SO

Description du projet :

Développement du filtre d’un masque de protection jetable destiné au grand public en appliquant l’electrospinning d’un polymère biodégradable.

Research team within HES-SO: Charmet Jérôme , Hengsberger Stefan , Marti Roger

Statut: Ongoing

2023

Flow rate-independent multiscale liquid biopsy for precision oncology

Scientific paper ArODES

Jie Wang, Robert Dallmann, Renquan Lu, Jing Yan, Jérôme Charmet

ACS Sensors, To be published.

Link to the publication

Summary:

Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management but typically suffer from low throughput, relative complexity, and postprocessing limitations. Here, we address these issues simultaneously by decoupling and independently optimizing the nano-, micro-, and macro-scales of an enrichment device that is simple to fabricate and operate. Unlike other affinity-based devices, our scalable mesh approach enables optimum capture conditions at any flow rate, as demonstrated with constant capture efficiencies, above 75% between 50 and 200 μL min–1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. We demonstrate its postprocessing capacity with the identification of potential responders to immune checkpoint inhibition (ICI) therapy and the detection of HER2 positive breast cancer. The results compare well with other assays, including clinical standards. This suggests that our approach, which overcomes major limitations associated with affinity-based liquid biopsies, could help improve cancer management.

2022

Multifunctional composite hydrogels for bacterial capture, growth/elimination, and sensing applications

Scientific paper ArODES

Andrea Dsouza, Chrystala Constatinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet, Rachel A. Hand

ACS Applied Materials & Interfaces, To be published

Link to the publication

Summary:

Hydrogels are crosslinked networks of hydrophilic polymer chains with a three-dimensional structure. Owing to their unique features, the application of hydrogels for bacterial/antibacterial studies and bacterial infection management has grown in importance in recent years. This trend is likely to continue due to the rise in bacterial infections and antimicrobial resistance. By exploiting their physiochemical characteristics and inherent nature, hydrogels have been developed to achieve bacterial capture and detection, bacterial growth or elimination, antibiotic delivery, or bacterial sensing. Traditionally, the development of hydrogels for bacterial/antibacterial studies have focused on achieving a single function such as antibiotic delivery, antibacterial activity, bacterial growth, or bacterial detection. However, recent studies demonstrate the fabrication of hydrogels that exhibit multifunctionality in the form of multifunctional hydrogels, where a single hydrogel is capable of performing more than one bacterial/antibacterial function or composite hydrogels consisting of a number of single functionalized hydrogels, which exhibit bacterial/antibacterial function synergistically. In this review, we first highlight the hydrogel features critical for bacterial studies and infection management. Then, we specifically address unique hydrogel properties, their surface/network functionalization, and mode of action for bacterial capture, adhesion/growth, antibacterial activity, and bacterial sensing respectively. Finally, we provide insights into different strategies for developing multifunctional hydrogels and how such systems can help tackle, manage, and understand bacterial infections and antimicrobial resistance. We also note that the strategies highlighted in this review can be adapted to other cell types and are therefore likely to find applications beyond the field of microbiology.

Flow rate independent multiscale liquid biopsy for precision oncology

Scientific paper ArODES

Jing Yan, Jie Wang, Robert Dallmann, Renquan Lu, Jérôme Charmet

arXiv.org,

Link to the publication

Summary:

Immunoaffinity-based liquid biopsies of circulating tumour cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues simultaneously by decoupling and independently optimising the nano-, micro- and macro-scales of a CTC enrichment device that is both simple to fabricate and operate. At its core is a scalable macroscale mesh with optimised micropores, nanofunctionalised with antibodies against cell surface proteins. Unlike other affinity-based liquid biopsies, optimum capture can be achieved independently of the flow rate, as demonstrated with constant capture efficiencies, above 75%, between 50–200 µL min-1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. To demonstrate its post-processing capabilities, we used immunofluorescence labelling to identify PD-L1+ CTCs in 36% of patients (n=33) as potential responders to immune checkpoint inhibition therapy. Finally, our device achieved an 80% positive match in the identification of HER2+ breast cancer (n=26) compared to clinical standard FISH on solid biopsy. The results suggest that our approach, which overcomes major limitations previously associated with affinity-based liquid biopsies, could provide a versatile tool to improve cancer management.

P10 rapid capture of uropathogenic bacteria and on-chip determination of antimicrobial resistance

Professional paper ArODES

Andrea Dsouza, Rachel Hand, Chrystala Constantinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet

JAC-Antimicrobial Resistance, 2022, vol. 4, suppl. no. 1

Link to the publication

Publications (sélection parmi >35 publications, >900 citations, h-index 16)

Scientific paper

Charmet Jérôme

Selection, 2022

Summary:

Liste complète ici

T. Kartanas et al., Analytical Chemistry 93, 2848, 2021
T. Kartanas et al., Applied Physics Letters, 116, 153702, 2020.
J. Charmet et al., Micromachines, 11, 135, 2020.
C. Bortolini, et al. Lab on a Chip, 19, 50, 2019.
R.S. Ruggeri, et al. Nature Communication 9, 3890, 2018
W.E. Arter, et al. Analytical Chemistry. 90, 10302, 2018.
J. Charmet, P. Arosio, T. P. J. Knowles, Journal of Molecular Biology. 430, 565, 2018.
T. Kartanas et al., Analytical Chemistry. 89, 11929, 2017.
M. Nikolka, et al., Nature Materials. 16, 356, 2017.
P. K. Challa, et al., Biomicrofluidics 11, 0141131, 2017.
J. Charmet, et al., Physical Review Applied. 5, 064016, 2016.
A. Prasad, J. Charmet, A. A. Seshia, Sensors and Actuators A Physical. 238, 207, 2016.
J. Charmet, et al., Applied Physics Letters. 105, 013502, 2014.

2022

Flow rate effect on THz-TDS of thin-film fluid in microfluidic device

Conference ArODES

Xuefei Ding, Jérôme Charmet, Emma Pickwell-McPherson

Proceedings of the 2022 47th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz), 28 August - 2 September 2022, Delft, Netherlands

Link to the conference

Summary:

Here we present a compact microfluidic device for thin-film water fluid measurements in reflection THz-TDS. We discuss the variables that need to be considered when utilizing this device in THz-TDS, and we find that different flow rates can cause subtle changes in the THz response.

Achievements

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Charmet Jérôme

Professeur-e HEs associé-e B

Main skills

Microfabrication

Biosensors

Microfluidics

Polymer technology

Functional materials

Professeur-e HEs associé-e B