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Prim Denis

Adjoint-e scientifique HES A

Main skills

Adjoint-e scientifique HES A

Phone: +41 58 606 86 28

Desktop: ENP.19.N108c

HES-SO Valais-Wallis - Haute Ecole d'Ingénierie
Rue de l'Industrie 23, 1950 Sion, CH

Faculty
Chimie et sciences de la vie

Main Degree Programme
Ingénierie des Sciences du vivant

Institute
Institut Sciences du vivant

BSc HES-SO en Ingénierie des sciences du vivant - HES-SO Valais-Wallis - Haute Ecole d'Ingénierie

Capteurs et instrumentations en bio-ingénierie

Completed

Swiss Symposium in Point-of-care Diagnostics 2020

AGP

Role: Collaborator

Requérant(e)s: VS - Institut Technologies du vivant

Financement: Divers clients

Description du projet : Swiss Symposium in Point-of-care Diagnostics 2020 [29.10.2020]

Research team within HES-SO: Prim Denis , Seppey Sherine , Pfeifer Marc Emil

Partenaires académiques: VS - Institut Technologies du vivant

Durée du projet: 01.01.2020 - 31.12.2020

Statut: Completed

Therapeutic drug monitoring for Personalized medicine - Nano-Tera.ch RTD

AGP

Role: Collaborator

Financement: VS - Institut Systèmes industriels; VS - Institut Technologies du vivant; Nano-Tera.CH; SEFRI (anc. OFFT)

Description du projet : (anc.TheraPer) Modern therapeutics must benefit from the development and large-scale implementation of convenient, user-friendly, miniaturized, integrated instruments enabling drug concentration monitoring and seamless pharmacokinetically guided dosage individualization.

Research team within HES-SO: Walpen Olivier , Vaccari Aldo , Prim Denis , Amoos Serge , Corthay François , Condemi Enrico , Geiser Martial , Maître Gilbert , Berthouzoz David , Truffer Frédéric , Segura Jean-Manuel , Pfeifer Marc Emil , Jacquemettaz Pascal , Mabillard Eric

Durée du projet: 01.11.2013 - 31.10.2017

Montant global du projet: 1'084'700 CHF

Statut: Completed

2024

Northwest to southwest – the conference reports of the 5th and 6th swiss symposium in point-of-care diagnostics held in Muttenz 2022 and Sion 2023

Professional paper ArODES

Marc Emil Pfeifer, Denis Prim

CHIMIA, 2024, 78, 4, 267-270

Link to the publication

2023

Biomarker-based diagnostics for mild traumatic brain injury (mTBI) at POC - rising to the challenges

Professional paper ArODES

Marc E. Pfeifer, Milica Jovic, Denis Prim

CHIMIA, 77, 7/8, 536

Link to the publication

Opening the black box of traumatic brain injury :

Scientific paper ArODES

a holistic approach combining human 3D neural tissue and an in vitro traumatic brain injury induction device

Céline Loussert-Fonta, Luc Stoppini, Yoan Neuenschwander, Ophélie Righini, Denis Prim, Cédric Schmidt, Marc O. Heuschkel, Loris Gomez Baisac, Milica Jovic, Marc E. Pfeifer, Jérôme Extermann, Adrien Roux

Frontiers in Neuroscience, 2023, vol. 17, article no. 1189615

Link to the publication

Summary:

Traumatic brain injury (TBI) is caused by a wide range of physical events and can induce an even larger spectrum of short- to long-term pathophysiologies. Neuroscientists have relied on animal models to understand the relationship between mechanical damages and functional alterations of neural cells. These in vivo and animal-based in vitro models represent important approaches to mimic traumas on whole brains or organized brain structures but are not fully representative of pathologies occurring after traumas on human brain parenchyma. To overcome these limitations and to establish a more accurate and comprehensive model of human TBI, we engineered an in vitro platform to induce injuries via the controlled projection of a small drop of liquid onto a 3D neural tissue engineered from human iPS cells. With this platform, biological mechanisms involved in neural cellular injury are recorded through electrophysiology measurements, quantification of biomarkers released, and two imaging methods [confocal laser scanning microscope (CLSM) and optical projection tomography (OPT)]. The results showed drastic changes in tissue electrophysiological activities and significant releases of glial and neuronal biomarkers. Tissue imaging allowed us to reconstruct the injured area spatially in 3D after staining it with specific nuclear dyes and to determine TBI resulting in cell death. In future experiments, we seek to monitor the effects of TBI-induced injuries over a prolonged time and at a higher temporal resolution to better understand the subtleties of the biomarker release kinetics and the cell recovery phases.

A novel point-of-care diagnostic prototype system for the simultaneous electrochemiluminescent sensing of multiple traumatic brain injury biomarkers

Scientific paper ArODES

Milica Jovic, Denis Prim, Ophélie Righini, David Tagan, Mélanie Stäuble, Marc Pignat, Steve Gallay, Martial Geiser, Marc E. Pfeifer

Sensors & Diagnostics, 2, 4, 964-975

Link to the publication

Summary:

Traumatic brain injuries (TBI) are typically acquired when a sudden violent event causes damage to the brain tissue. A high percentage (70–85%) of all TBI patients are suffering from mild TBI (mTBI), which is often difficult to detect and diagnose with standard imaging tools (MRI, CT scan) due to the absence of significant lesions and specific symptoms. Recent studies suggest that a screening test based on the measurement of a protein biomarker panel directly from a patient's blood can facilitate mTBI diagnosis. Herein, we report a novel prototype system designed as a precursor of a future hand-held point-of-care (POC) diagnostic device for the simultaneous multi-biomarker sensing, employing a microarray-type spatially resolved electrochemiluminescence immunoassay (SR-ECLIA). The small tabletop prototype consists of a screen-printed electrode compartment to conduct multi-analyte ECL sandwich assays, a potentiostat module and a light collection module, all integrated into a compact 3D-printed housing (18.2 × 16.5 × 5.0 cm), as well as an sCMOS detector. Based on this design concept, further miniaturization, system integration, performance optimization and clinical evaluation shall pave the way towards the development of a portable instrument for use at the site of accident and healthcare. To demonstrate the system's feasibility, current performance and efficiency, the simultaneous detection of three mTBI biomarkers (GFAP, h-FABP, S100β) in 50% serum was achieved in the upper pg mL−1 range. The proposed device is amenable to the detection of other biomarker panels and thus could open new medical diagnostic avenues for sensitive multi-analyte measurements with low-volume biological sample requirements

2022

Fluorescence-polarization immunoassays within glass fiber micro-chambers enable tobramycin quantification in whole blood for therapeutic drug monitoring at the point of care

Scientific paper ArODES

Elena-Diana Bojescu, Denis Prim, Marc Pfeifer, Jean-Manuel Segura

Analytica Chimica Acta, 2022, vol. 1225, article no. 340240

Link to the publication

Summary:

Many therapeutic drugs require monitoring of their concentration in blood followed by dose adjustments in order to ensure efficacy while minimizing adverse effects. It would be highly desirable to perform such measurements rapidly and with reduced sample volumes to support point-of-care testing. Here, we demonstrate that the concentration of small therapeutics can be determined in whole blood within paper-like membranes using Fluorescence Polarization Immunoassay (FPIA). Different types of paper-like materials such as glass microfibers, cellulose and filter paper were investigated for artefacts such as scattering or autofluorescence. Accurate determination of the fluorescence polarization of red-emitting fluorophores at sub-nanomolar concentrations was feasible within glass fiber membranes. This enabled the development of a competitive immunoassay for the quantification of the antibiotic tobramycin using only 1 μL of plasma in glass fiber micro-chambers. Furthermore, the same membrane was used for transversal separation of blood cells followed by accurate FPIA read-out at the bottom part of the micro-chamber. For quantification of tobramycin, 1 μL of whole blood was incubated with the immunoassay reagents during only 3 min before deposition in the micro-chamber and analysis. Within the therapeutic window, coefficients of variation were around 20% and recoveries between 80 and 105%. Owing to the simplified procedure requiring no centrifugation, the reduced blood sample volume and the rapid analysis time, we envision that this novel method supports the performance of therapeutic drug monitoring directly at the point of care.

2021

More publications on my ResearchGate profile

Scientific paper

Plus de publications sur mon profil ResearchGate

Prim Denis

Research Gate, 2021

Link to the publication

2020

Analytical platforms at swiss universities of applied sciences

Scientific paper ArODES

Christian Berchtold, Verena Christen, Jean-Pascal Bourgeois, Michal Dabros, Olivier Nicolet, Cyril Portmann, Samuel Roth, Olivier Vorlet, Caspar Demuth, Susanne Kern, Chahan Yeretzian, Anika Hoffmann, Franka Kalman, Nadia Marcon, Marc E. Pfeifer, Umberto Piantini, Denis Prim, Jean-Manuel Segura, Mathieu Zollinger, Götz Schlotterbeck

CHIMIA International Journal for Chemistry, 2020, vol. 74, no. 7/8, pp. 618-628

Link to the publication

Summary:

Numerous projects and industrial and academic collaborations benefit from state-of-the-art facilities and expertise in analytical chemistry available at the Swiss Universities of Applied Sciences. This review summarizes areas of expertise in analytical sciences at the University of Applied Sciences and Arts Northwestern Switzerland (FHNW), the University of Applied Sciences and Arts Western Switzerland (HES-SO), and the Zurich University of Applied Sciences (ZHAW). We briefly discuss selected projects in different fields of analytical sciences

2016

Rapid and straightforward quantification of recombinant proteins using fluorescence polarization

Scientific paper ArODES

Enrico Condemi, Denis Prim, Rebecca Brönnimann, Jonathan Fusco, Simon Crelier, Olimpia Mamula Steiner, Jean-Manuel Segura

CHIMIA International Journal for Chemistry, 2016, vol. 70, no. 10, p. 731

Link to the publication

Manufacturing of peptide microarrays based on catalyst-free click chemistry

Book chapter ArODES

Denis Prim, Marc E. Pfeifer

Dans Chiari, Marcella, Cretich, Marina, Methods in Molecular Biology (pp. 157-166). 2016, New York : Humana Press

Link to the publication

Summary:

Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.

2015

Identification of bioaccessible and uptaken phenolic compounds from strawberry fruits in in vitro digestion/Caco-2 absorption model

Scientific paper ArODES

Agnieszka Kosinska-Cagnazzo, Sascha Diering, Denis Prim, Wilfried Andlauer

Food Chemistry, 2015, vol. 170, pp. 288-294

Link to the publication

Summary:

Strawberry fruits are highly valued for their taste and nutritional value. However, results describing the bioaccessibility and intestinal absorption of phenolic compounds from strawberries are still scarce. In our study, a combined in vitro digestion/Caco-2 absorption model was used to mimic physiological conditions in the gastrointestinal track and identify compounds transported across intestinal epithelium. In the course of digestion, the loss of anthocyanins was noted whilst pelargonidin-3-glucoside remained the most abundant compound, amounting to nearly 12 mg per 100 g of digested strawberries. Digestion increased the amount of ellagic acid available by nearly 50%, probably due to decomposition of ellagitannins. Only trace amounts of pelargonidin-3-glucoside were found to be absorbed in the intestine model. Dihydrocoumaric acid sulphate and p-coumaric acid were identified as metabolites formed in enterocytes and released at the serosal side of the model.

2013

ADIBO-based “Click” chemistry for diagnostic peptide micro-array fabrication :

Scientific paper ArODES

physicochemical and assay characteristics

Denis Prim, Fabien Rebeaud, Vincent Cosandey, Roger Marti, Philippe Passeraub, Marc E. Pfeifer

Molecules, 2013, vol. 18, no. 8, pp. 9833-9849

Link to the publication

Summary:

Several azide-derivatized and fluorescently-labeled peptides were immobilized on azadibenzocyclooctyne (ADIBO)-activated slide surfaces via a strain-promoted alkyne-azide cycloaddition (SPAAC) reaction revealing excellent immobilization kinetics, good spot homogeneities and reproducible fluorescence signal intensities. A myc-peptide micro-array immunoassay showed an antibody limit-of-detection (LOD) superior to a microtiter plate-based ELISA. Bovine serum albumin (BSA) and dextran covalently attached via “click” chemistry more efficiently reduced non-specific binding (NSB) of fluorescently-labeled IgG to the microarray surface in comparison to immobilized hexanoic acid and various types of polyethylene glycol (PEG) derivatives. Confirmation of these findings via further studies with other proteins and serum components could open up new possibilities for human sample and microarray platform-based molecular diagnostic tests.

Phenolic compounds profile of strawberry fruits of Charlotte cultivar

Scientific paper ArODES

Agnieszka Kosinska, Sascha Diering, Denis Prim, Julien Héritier, Wilfried Andlauer

Journal of Berry Research, 2013, vol. 3, pp. 15-23

Link to the publication

Summary:

The profile of phenolic compounds in strawberry fruits varies significantly among cultivars. High performance liquid chromatography with diode array detection coupled to electrospray ionization mass spectrometric detection with positive and negative modes of ionization was employed to identify phenolic compounds in extract of strawberries from Charlotte cultivar. This is the first time phenolic profile of Charlotte cultivar has been characterized. The fruits contained phenolics belonging to six groups: anthocyanins, ellagic acid and its conjugates, gallotannins, flavonols, flavanols and hydroxycinnamic acid derivatives. The presence of pentagalloyl glucose in strawberries fruits was reported for the first time.

ADIBO-Based “Click” Chemistry for Diagnostic Peptide Micro-Array Fabrication: Physicochemical and Assay Characteristics

Scientific paper

Marti Roger, Pfeifer Marc Emil, Prim Denis, Fabien Rebeaud, Vincent Cosandey, Passeraub Philippe,

Molecules, 2013 , vol. 18, pp. 9833-9849

Link to the publication

Summary:

2012

Construction of a peptide microarray for auto-anti-body detection

Scientific paper ArODES

Vincent Cosandey, Fabien Debrot, Jérémy Kaeser, Roger Marti, Philippe Passeraub, Jannick Pétremand, Denis Prim, Marc E. Pfeifer

CHIMIA International Journal for Chemistry, 2012, vol. 66, no. 10, pp. 803-806

Link to the publication

Summary:

Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).

Construction of a Peptide Microarray for Auto-antibody Detection

Scientific paper

Marti Roger, Vincent Cosandey, Fabien Debrot, Jérémy Kaeser, Passeraub Philippe, Jannick Pétremand, Prim Denis, Pfeifer Marc Emil

Chimia, 2012 , vol. 66, pp. 803-806

Link to the publication

Summary:

2011

Coupling of a microfluidic mixer to a Fourier-transform infrared spectrometer for protein-conformation studies

Scientific paper ArODES

Denis Prim, Simon Crelier, Jean-Manuel Segura

CHIMIA International Journal for Chemistry, 2011, vol. 65, no. 10, pp. 815-816

Link to the publication

Summary:

The biological properties of a protein critically depend on its conformation, which can vary as a result of changes in conditions such as pH or following the addition of various substances. Being able to reliably assess the quality of protein structures under various conditions is therefore of crucial importance. Infrared (IR) spectroscopy of the Amide I band of proteins is a powerful method for the determination of protein conformations and further allows the analysis of continuously flowing solutions of the target molecule. Here, a commercial Fourier-transform infrared spectrometer was coupled to a microfluidic mixer to allow the on-line monitoring of protein conformation under varying conditions. The validity of the concept was demonstrated by continuously recording the variations of the IR spectrum of poly-L-lysine resulting from repetitive, pH-induced conformational changes.

2009

Fluorometric method to assess lipase inhibition activity

Scientific paper ArODES

Wilfried Andlauer, Patrice Prunier, Denis Prim

CHIMIA International Journal for Chemistry, 2009, vol. 63, no. 12, pp. 897-899

Link to the publication

Summary:

Obesity and excess weight have become serious health problems in our developed societies today. Increased blood pressure, blood glucose levels and abnormal blood lipids are frequent consequences. Inhibition of digestive enzymes by pharmacological or nutritional intervention are one avenue to be considered to treat this population. In the present study a robust assay to screen biologically active materials for their ability to inhibit pancreatic lipase, the most important enzyme in fat digestion, has been developed. Methyl-umbelliferyl butyrate was used as an artificial substrate, enabling assessment of lipase activity via specific fluorescence emission. Applicability of the assay was shown by assessment of lipase inhibition activity of wild plants from Switzerland and France. Testing showed some plants to have a high inhibition rate of about 70%. In further projects, this lipase inhibition assay could be used for a scientific proof of biological activity of raw materials with the intention to develop functional foods for weight reduction.

Achievements

PEOPLE@HES-SO
Directory and Skills inventory

Prim Denis

Adjoint-e scientifique HES A

Main skills

Bio-Assay development

Bioanalytique

Analytical Chemistry

Project management

Adjoint-e scientifique HES A

PEOPLE@HES-SO Directory and Skills inventory

Prim Denis

Adjoint-e scientifique HES A

Main skills

Bio-Assay development

Bioanalytique

Analytical Chemistry

Project management

Adjoint-e scientifique HES A

PEOPLE@HES-SO
Directory and Skills inventory