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PEOPLE@HES-SO - Verzeichnis der Mitarbeitenden und Kompetenzen
PEOPLE@HES-SO - Verzeichnis der Mitarbeitenden und Kompetenzen

PEOPLE@HES-SO
Verzeichnis der Mitarbeitenden und Kompetenzen

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Heuschkel Marc

Heuschkel Marc

Adjoint-e scientifique ou artistique HES

Hauptkompetenzen

Microfabrication

Microsystems

Biological interfaces

Biochip

Electrophysiology

Microfluidics

Sensors

  • Kontakt

  • Lehre

  • Publikationen

Hauptvertrag

Adjoint-e scientifique ou artistique HES

Büro: Biotech - B3.02 197.136

Haute école du paysage, d'ingénierie et d'architecture de Genève
Rue de la Prairie 4, 1202 Genève, CH
hepia
Bereich
Technique et IT
Hauptstudiengang
Microtechniques
BSc HES-SO en Microtechniques - Haute école du paysage, d'ingénierie et d'architecture de Genève
  • Microtechnique

2024

Versatile micro-electrode array to monitor human iPSC derived 3D neural tissues at air-liquid interface
Wissenschaftlicher Artikel ArODES

Luc Stoppini, Marc Heuschkel, Céline Loussert-Fonta, Loris Gomez Baisac, Adrien Roux

Frontiers in Cellular Neuroscience,  2024, 18

Link zur Publikation

Zusammenfassung:

Engineered 3D neural tissues made of neurons and glial cells derived from human induced pluripotent stem cells (hiPSC) are among the most promising tools in drug discovery and neurotoxicology. They represent a cheaper, faster, and more ethical alternative to in vivo animal testing that will likely close the gap between in vitro animal models and human clinical trials. Micro-Electrode Array (MEA) technology is known to provide an assessment of compound effects on neural 2D cell cultures and acute tissue preparations by real-time, non-invasive, and long-lasting electrophysiological monitoring of spontaneous and evoked neuronal activity. Nevertheless, the use of engineered 3D neural tissues in combination with MEA biochips still involves series of constraints, such as drastically limited diffusion of oxygen and nutrients within tissues mainly due to the lack of vascularization. Therefore, 3D neural tissues are extremely sensitive to experimental conditions and require an adequately designed interface that provides optimal tissue survival conditions. A well-suited technique to overcome this issue is the combination of the Air-Liquid Interface (ALI) tissue culture method with the MEA technology. We have developed a full 3D neural tissue culture process and a data acquisition system composed of high-end electronics and novel MEA biochips based on porous, flexible, thin-film membranes integrating recording electrodes, named as “Strip-MEA,” to allow the maintenance of an ALI around the 3D neural tissues. The main motivation of the porous MEA biochips development was the possibility to monitor and to study the electrical activity of 3D neural tissues under different recording configurations, (i) the Strip-MEA can be placed below a tissue, (ii) or by taking advantage of the ALI, be directly placed on top of the tissue, or finally, (iii) it can be embedded into a larger neural tissue generated by the fusion of two (or more) tissues placed on both sides of the Strip-MEA allowing the recording from its inner part. This paper presents the recording and analyses of spontaneous activity from the three positioning configurations of the Strip-MEAs. Obtained results are discussed with the perspective of developing in vitro models of brain diseases and/or impairment of neural network functioning.

2023

Opening the black box of traumatic brain injury: a holistic approach combining human 3D neural tissue and an in vitro traumatic brain injury induction device
Wissenschaftlicher Artikel

Céline Loussert-Fonta, Stoppini Luc, Yoan Neuenschwander, Ophélie Righini, Prim Denis, Cédric Schmidt, Heuschkel Marc, Loris Gomez Baisac, Jovic Milica, Pfeifer Marc Emil, Extermann Jérôme, Roux Adrien

Frontiers in Neuroscience, 2023 , vol.  17, no  1189615

Link zur Publikation

Opening the black box of traumatic brain injury: a holistic approach combining human 3D neural tissue and an in vitro traumatic brain injury induction device
Wissenschaftlicher Artikel

Céline Loussert-Fonta, Stoppini Luc, Yoan Neuenschwander, Ophélie Righini, Denis Prim, Schmidt Cédric, Heuschkel Marc, Loris Gomez Baisac, Milica Jović, Marc E. Pfeifer, Extermann Jérôme, Roux Adrien

Front. Neurosci., 2023 , vol.  17:1189615

Link zur Publikation

Zusammenfassung:

Traumatic brain injury (TBI) is caused by a wide range of physical events and can induce an even larger spectrum of short- to long-term pathophysiologies. Neuroscientists have relied on animal models to understand the relationship between mechanical damages and functional alterations of neural cells. These in vivo and animal-based in vitro models represent important approaches to mimic traumas on whole brains or organized brain structures but are not fully representative of pathologies occurring after traumas on human brain parenchyma. To overcome these limitations and to establish a more accurate and comprehensive model of human TBI, we engineered an in vitro platform to induce injuries via the controlled projection of a small drop of liquid onto a 3D neural tissue engineered from human iPS cells. With this platform, biological mechanisms involved in neural cellular injury are recorded through electrophysiology measurements, quantification of biomarkers released, and two imaging methods [confocal laser scanning microscope (CLSM) and optical projection tomography (OPT)]. The results showed drastic changes in tissue electrophysiological activities and significant releases of glial and neuronal biomarkers. Tissue imaging allowed us to reconstruct the injured area spatially in 3D after staining it with specific nuclear dyes and to determine TBI resulting in cell death. In future experiments, we seek to monitor the effects of TBI-induced injuries over a prolonged time and at a higher temporal resolution to better understand the subtleties of the biomarker release kinetics and the cell recovery phases.

2021

SpikeOnChip : A Custom Embedded Platform for Neuronal Activity Recording and Analysis
Wissenschaftlicher Artikel

Rick Wertenbroek, Yann Thoma, Flavio Maurizio Mor, Sara Grassi, Heuschkel Marc, Roux Adrien, Stoppini Luc

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, 2021 , vol.  15, no  4, pp.  743-755

Link zur Publikation

Zusammenfassung:

In this paper we present SpikeOnChip, a custom embedded platform for neuronal activity recording and online analysis. The SpikeOnChip platform was developed in the context of automated drug testing and toxicology assessments on neural tissue made from human induced pluripotent stem cells. The system was developedwith the following goals: to be small, autonomous and low power, to handle micro-electrode arrays with up to 256 electrodes, to reduce the amount of data generated from the recording, to be able to do computation during acquisition, and to be customizable. This led to the choice of a Field Programmable Gate Array System-On-Chip platform. This paper focuses on the embedded system for acquisition and processing with key features being the ability to record electrophysiological signals from multiple electrodes, detect biological activity on all channels online for recording, and do frequency domain spectral energy analysis online on all channels during acquisition.Development methodologies are also presented. The platform is finally illustrated in a concrete experiment with bicuculline being administered to grown human neural tissue through microfluidics, resulting in measurable effects in the spike recordings and activity. The presented platformprovides a valuable new experimental instrument that can be further extended thanks to the programmable hardware and software.

2018

Development and Characterization of PEDOT:PSS/Alginate Soft Microelectrodes for Application in Neuroprosthetics
Wissenschaftlicher Artikel

Laura Ferlauto, Antonio Nunzio D'Angelo, Paola Vagni, Marta Leccardi, Mor Flavio, Estelle Cuttaz, Heuschkel Marc, Stoppini Luc, D Ghezzi

Frontiers in Neuroscience, Neural Technology, 2018 , vol.  12, no  648

Link zur Publikation

Zusammenfassung:

Reducing the mechanical mismatch between the stiffness of a neural implant and the softness of the neural tissue is still an open challenge in neuroprosthetics. The emergence of conductive hydrogels in the last few years has considerably widened the spectrum of possibilities to tackle this issue. Nevertheless, despite the advancements in this field, further improvements in the fabrication of conductive hydrogel-based electrodes are still required. In this work, we report the fabrication of a conductive hydrogel-based microelectrode array for neural recording using a hybrid material composed of poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate), and alginate. The mechanical properties of the conductive hydrogel have been investigated using imaging techniques, while the electrode arrays have been electrochemically characterized at each fabrication step, and successfully validated both in vitro and in vivo. The presence of the conductive hydrogel, selectively electrodeposited onto the platinum microelectrodes, allowed achieving superior electrochemical characteristics, leading to a lower electrical noise during recordings. These findings represent an advancement in the design of soft conductive electrodes for neuroprosthetic applications.

2016

An evaluation of extracellular MEA versus optogenetic stimulation of cortical neurons
Wissenschaftlicher Artikel

Vanessa Maybeck, Jan Schnitker, Wenfang Li, Heuschkel Marc, Andreas Offenhäusser

Biomed. Phys. Eng. Express, 2016 , vol.  2, no  055017

Link zur Publikation

Zusammenfassung:

Objective. The importance of extracellular neural stimulation has driven the development of multiple technologies. Of growing importance is accurately stimulating single neurons in dense networks. It is unlikely that one approach is best for all applications, however comparisons between methods are lacking. We aim to show the strengths and suitable applications for two tools; micro-electrode array (MEA) stimulation and optogenetics. Approach. We compare MEA-based electrical stimulation to Channelrhodopsin 2 based optogenetic stimulation of dissociated cortical neurons in vitro. Effectivity is compared based on stimulation success rate, spatial and temporal accuracy, and reproducibility. We discuss how necessities of each method may limit performance in each category. Main Results. MEA stimulation outperformed optogenetic stimulation in the speed with which an action potential could be generated. The relation between the size of the stimulating point (electrode or illumination spot) and the area of stimulated tissue was similar in both methods. However, technical difficulties in maintaining low impedance from very small electrodes allows higher spatial specificity in optogenetic stimulation. If simultaneous recording and stimulation are desired, MEA stimulation artifacts were far more impairing than light induce artifacts on MEA recordings. Significance. The like versus like comparison of stimulation technologies provides an incomplete evaluation tool for researchers desiring to apply these technologies. This comparison highlights advantages for specific applications and should promote more cross-topic evaluations.

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