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Charmet Jérôme

Professeur-e HEs associé-e B

Hauptkompetenzen

Professeur-e HEs associé-e B

Haute Ecole Arc - Ingénierie
Espace de l'Europe 11, 2000 Neuchâtel, CH

BSc HES-SO en Microtechniques - Haute Ecole Arc - Ingénierie

Chimie générale
Biophysique
Biomatériaux et biocompatibilité
Technologie médicales

MSc in Biomedical Engineering - Universität Bern

Intelligent Implants and Surgical Instruments

Laufend

HYPERCELL

Rolle: Hauptgesuchsteller/in

Financement: FNS - Health and Wellbeing call

Description du projet :

Adoptive T cell therapy (aTcT) is a highly promising type of immunotherapy used to treat several malignancies. This type of therapy is particularly promising for non-small cell lung cancer (NSCLC), the deadliest cancer worldwide. However, its efficacy remains very low. In addition, like many other immunotherapies, aTcT is expensive, not effective for every patient and may have serious side-effects.

Therefore, there is a need for fast, personalized and minimally disruptive assays to measure the efficacy of adoptive T cell therapy, which is our main objective.

The project is split in two distinct specific objectives:

1) Development and validation of a single device, called the HYPERCELL chip that will allow for the evaluation of the efficacy of autologous adoptive-cells therapy at the single cell level. The unique chip will be composed of a re-usable control module, a disposable liquid biopsy assay and cytotoxicity assay modules, to deliver a simple, fast and minimally disruptive assay.

2) Study and understanding process-dependent shear stress and its effect on cell viability and function ex vivo. The insights gained from this study will be applicable to a wide range of assays, beyond the HYPERCELL project itself.

By providing an integrated solution that allows for seamless evaluation of T cells therapies on patients derived cells, the HYPERCELL chip has the potential to improve their outcomes. Indeed, this device that will be self-contained relies on existing laboratory workflows and equipment, it is therefore expected to be easily adopted in clinical environment.

Forschungsteam innerhalb von HES-SO: Charmet Jérôme

Partenaires académiques: Cleo Goyvaerts, VUB, Belgium; Alexandre Kuhn, HES-SO Valais

Durée du projet: 01.10.2024 - 30.09.2027

Montant global du projet: 1'010'261 CHF

Statut: Laufend

ELUSIVE

Rolle: Hauptgesuchsteller/in

Financement: HES-SO

Description du projet :

Robust, mechanically enhanced, and controllable/triggerable degradation solution for sustainable PCB.

Forschungsteam innerhalb von HES-SO: Charmet Jérôme

Partenaires professionnels: Pascal Nussbaum, CSEM; Pierre Blain, Sonceboz SA

Durée du projet: 01.04.2024 - 31.03.2025

Montant global du projet: 55'000 CHF

Statut: Laufend

DNAMIC

Rolle: Mitgesuchsteller/in

Requérant(e)s: Ignas Galminas, Genomika, LT

Financement: H2020 - EIC Pathfinder

Description du projet :

We live in a society that produces and requires more and more data every year. It has become apparent that our current data storage strategies are not adequate to meet this increasing demand.

The quest for robust, high-density, sustainable, and economically viable data storage solutions has highlighted the enormous potential and suitability of DNA data storage.

However, to date, none of the solutions have considered a comprehensive vision of anchoring this technology in standardised archival frameworks that will support long-term storage. Besides, most DNA data storage applications are limited to specialist laboratories due to complex (and expensive) protocols.

Our project entitled DNAMIC (DNA Microfactory for Autonomous Archiving) proposes an autonomous solution based around a low energy consumption microfactory that will be developed for end-to-end DNA data archiving (from encoding to decoding via synthesis, storage, quality control and sequencing among others).

The microfactory is interoperable and future proof thanks to technology block that can be easily modified or replaced. Our solution will be compliant with the Open Archival Information System (OAIS) reference model (ISO 14721). To enable the implementation of disaster recovery strategies, critical for long term storage, we propose a novel dual-level encoding scheme. This approach allows the development of self-contained data that include easily changeable metadata, and unalterable data.

Forschungsteam innerhalb von HES-SO: Charmet Jérôme , Neuenschwander Patrick , Serex Florian , Beurret Stéphane , Remonnay Valentin , Salvadé Yves , Angeloni Suter Silvia , Wenger Samuel , Béguin Vincent

Partenaires académiques: Fritz Simmel, TUM, DE; Thomas Heinis, Imperial College, UK; Renaldas Raisutis, KTU, LT; Pierre-Yves Burgi, Université de Genève

Partenaires professionnels: Ignas Galminas, Genomika, LT; Martin Jost, Kilobaser, Mabeal, AT

Durée du projet: 01.10.2023 - 30.09.2026

Montant global du projet: 4'951'890 CHF

Url des Projektstandortes: https://dnamic.org

Statut: Laufend

BiModal Sensor

Rolle: Mitgesuchsteller/in

Requérant(e)s: Ender Yildirim, METU, Turkey

Financement: TUBITAK

Description du projet :

Dual sensor for pleural effusion

Forschungsteam innerhalb von HES-SO: Charmet Jérôme

Partenaires académiques: Ender Yildirim, METU, Turkey

Statut: Laufend

Public Mask

Rolle: Mitgesuchsteller/in

Financement: HES-SO

Description du projet :

Développement du filtre d’un masque de protection jetable destiné au grand public en appliquant l’electrospinning d’un polymère biodégradable.

Forschungsteam innerhalb von HES-SO: Charmet Jérôme , Hengsberger Stefan , Marti Roger

Statut: Laufend

Abgeschlossen

Misc projets

Rolle: Hauptgesuchsteller/in

Description du projet :

Projets de recherche en tant que Professeur Associé à l'Université de Warwick.

(total > £2 mio. entre 2016-2021 en tant qu’investigateur principal)

• Feeling the force: Enhancing OOAC capability for biomechanical assessment and application. UK Research and Innovation (Co-investigateur, £23k), mars 2021.
• Organ-on-chip platform for TEER imaging, UK Research and Innovation (£30k), janvier 2021.
• Capacity Building for Prototyping Compatible Injection Moulding, High Value Manufacturing (£236k, trois partenaires industriels), mars 2020.
• Identification of metastatic biomarkers in nasopharyngeal carcinoma (NPC) to improve clinical management, Global Challenges Research Fund, (Co-investigateur, £50k), décembre 2019.
• MICRA Commercialisation Development Grant Award (£19k, deux partenaires industriels), dé-cembre 2019.
• Engineering Cardiovascular Systems using Protein Scaffolds, Monash-Warwick Alliance, Acceler-ator project (£247k), juillet 2019.
• Blood-brain-barrier on a chip to study the clinical relevance of circadian timing of anticancer drugs. Royal Society RGS\R2\180434 (£19k), mars 2019.
• Microfabrication suite. Capital Award for ECR. Engineering and Physical Science Research Coun-cil (EPSRC) EP/S017887/1 (£80k), janvier 2019.
• PleuSense, Differential diagnosis of pleural effusion, Institutional Links grant, ID 352360246, under the Newton-Katip Celebi Fund partnership, (£288k, deux partenaires industriels), février 2018.
• mPatch, mobile device for fitness and health monitoring. High Value Manufacturing Catapult (£213k, deux partenaires industriels), octobre 2017.
• Integrated platform with MEMS cantilever for the early detection of prostate cancer using dry mass sensing. EPSRC EP/R00403X/1 (£126k, un partenaire industriel), octobre 2017.
• Diamond Light Source beamtime, Beamline B22, B23 (67 shifts, ~£580k, deux partenaires indus-triels), depuis mars 2017.
• Mandats Industriels (£205k), depuis janvier 2017.
• University of Warwick; Research development fund, Global Fund partnership, Global Research Priority (total: £40k), depuis mars 2017.

Forschungsteam innerhalb von HES-SO: Charmet Jérôme

Durée du projet: - 14.02.2022

Montant global du projet: 2'500'000 CHF

Statut: Abgeschlossen

2024

Investigating demoulding characteristics of material jetted rapid mould inserts for microinjection moulding using inline monitoring and surface metrology

Wissenschaftlicher Artikel ArODES

Mert Gülçür, Dmitry Isakov, Jérôme Charmet, Gregory J. Gibbons

Rapid Prototyping Journal, 2024, 30, 7, 1322-1336

Link zur Publikation

Zusammenfassung:

This study aims to investigate the demoulding characteristics of material-jetted rapid mould inserts having different surface textures for micro-injection moulding using in-line measurements and surface metrology. Material-jetted inserts with the negative cavity of a circular test product were fabricated using different surface finishes and printing configurations, including glossy, matte and vertical settings. In-line measurements included the recording of demoulding forces at 10 kHz, which was necessary to capture the highly-dynamic characteristics. A robust data processing algorithm was used to extract reliable demoulding energies per moulding run. Thermal imaging captured surface temperatures on the inserts after demoulding. Off-line measurements, including focus variation microscopy and scanning electron microscopy, compared surface textures after a total of 60 moulding runs. A framework for capturing demoulding energies from material-jetted rapid tools was demonstrated and compared to the literature. Glossy surfaces resulted in significantly reduced demoulding forces compared to the industry standard steel moulds in the literature and their material-jetted counterparts. Minimal changes in the surface textures of the material-jetted inserts were found, which could potentially permit their prolonged usage. Significant correlations between surface temperatures and demoulding energies were demonstrated. The research presented here addresses the very topical issue of demoulding characteristics of soft, rapid tools, which affect the quality of prototyped products and tool durability. This was done using state-of-the-art, high-speed sensing technologies in conjunction with surface metrology and their durability for the first time in the literature.

Electrolyte-gated organic field-effect transistors with high operational stability and lifetime in practical electrolytes

Wissenschaftlicher Artikel ArODES

Dimitrios Simatos, Mark Nikolka, Jérôme Charmet, Leszek J. Spalek, Zenon Toprakcioglu, Ian E. Jacobs, Ivan B. Dimov, Guillaume Schweicher, Mi Jung Lee, Carmen M. Fernández-Posada, Duncan J. Howe, Tuuli A. Hakala, Lianne W. Y. Roode, Vincenzo Pecunia, Thomas P. Sharp, Weimin Zhang, Mariam Alsufyani, Iain McCulloch, Tuomas, P. J. Knowles, Henning Sirringhaus

SmartMat, 2024, e1291, To be published

Link zur Publikation

Zusammenfassung:

A key component of organic bioelectronics is electrolyte-gated organic field-effect transistors (EG-OFETs), which have recently been used as sensors to demonstrate label-free, single-molecule detection. However, these devices exhibit limited stability when operated in direct contact with aqueous electrolytes. Ultrahigh stability is demonstrated to be achievable through the utilization of a systematic multifactorial approach in this study. EG-OFETs with operational stability and lifetime several orders of magnitude higher than the state of the art have been fabricated by carefully controlling a set of intricate stability-limiting factors, including contamination and corrosion. The indacenodithiophene-co-benzothiadiazole (IDTBT) EG-OFETs exhibit operational stability that exceeds 900 min in a variety of widely used electrolytes, with an overall lifetime exceeding 2 months in ultrapure water and 1 month in various electrolytes. The devices were not affected by electrical stress-induced trap states and can remain stable even in voltage ranges where electrochemical doping occurs. To validate the applicability of our stabilized device for biosensing applications, the reliable detection of the protein lysozyme in ultrapure water and in a physiological sodium phosphate buffer solution for 1500 min was demonstrated. The results show that polymer-based EG-OFETs are a viable architecture not only for short-term but also for long-term biosensing applications.

High-performance, easy-to-fabricate, nanocomposite heater for life sciences and biomedical applications

Wissenschaftlicher Artikel ArODES

Yudan Whulanza, Husein Anmar, Deni Haryadi, Azizah Intan Pangesty, Widoretno Widoretno, Didik Tulus Subekti, Jérôme Charmet

Polymers, 2024, 16, 8, 1164

Link zur Publikation

Zusammenfassung:

Microheaters are used in several applications, including medical diagnostics, synthesis, environmental monitoring, and actuation. Conventional microheaters rely on thin-film electrodes microfabricated in a clean-room environment. However, low-cost alternatives based on conductive paste electrodes fabricated using printing techniques have started to emerge over the years. Here, we report a surprising effect that leads to significant electrode performance improvement as confirmed by the thorough characterization of bulk, processed, and conditioned samples. Mixing silver ink and PVA results in the solubilization of performance-hindering organic compounds. These compounds evaporate during heating cycles. The new electrodes, which reach a temperature of 80 °C within 5 min using a current of 7.0 A, display an overall 42% and 35% improvement in the mechanical (hardness) and electrical (resistivity) properties compared to pristine silver ink electrodes. To validate our results, we use the composite heater to amplify and detect parasite DNA from Trypanosoma brucei, associated with African sleeping sickness. Our LAMP test compares well with commercially available systems, confirming the excellent performance of our nanocomposite heaters. Since their fabrication relies on well-established techniques, we anticipate they will find use in a range of applications.

2023

Tunable and biodegradable poly(ester amide)s for disposable facemasks

Wissenschaftlicher Artikel ArODES

Esteban Alvarez Seoane, Alessandro Cattaneo, Fabien Neuenschwander, Lucien Blanchard, Tatiana Nogueira Matos, Laure Jeandupeux, Gianni Fiorucci, Maryam Tizgadam, Kelly Tran, Pierre-Louis Sciboz, Luce Albergati, Jérôme Charmet, Roger Marti, Stefan Hengsberger

Macromolecular Materials and Engineering, 2300375

Link zur Publikation

Zusammenfassung:

The widespread use of disposable facemasks during the COVID-19 pandemic has led to environmental widespread concern due to microplastic pollution. Biodegradable disposable facemasks are a first step to reducing the environmental impact of pandemics. In this paper we present high-performance facemask components based on novel poly(ester amide) (PEA) grades synthesized from bio-sourced materials and processed into non-woven facemask components. PEA based polymers present an excellent compromise between mechanical performance and biodegradability. Importantly, the properties of the PEA can easily be tuned by changing the ratio of the ester and amides, or variation of diol and diacid part. We synthesized seven polymers which we optimized for biodegradability and processability. Among them, two grades combined electrospinning process compatibility with full degradation within 35 days, using a normalized biodegradation test. The ultra-thin filters thus developed were evaluated for performance on a custom-made characterization bench. The filters achieved a microparticle capture efficiency and breathability comparable to commercial filters. Another PEA grade was optimized to reach optimal visco-thermal properties that made it compatible with solvent-free melt-spinning process as demonstrated with continuous fibres production. Overall, our environmentally friendly solution paves the way for the fabrication of high-performance fibres with excellent biodegradability for the next generation facemasks.

Effects of processing-induced contamination on organic electronic devices

Wissenschaftlicher Artikel ArODES

Dimitrios Simatos, Ian E. Jacobs, Illia Dobryden, Malgorzata Nguyen, Achilleas Savva, Deepak Venkateshvaran, Mark Nikolka, Jérôme Charmet, Leszek J. Spalek, Mindaugus Gicevicius, Youcheng Zhang, Guillaume Schweicher, Duncan J. Howe, Sarah Ursel, John Armitage, Ian B. Dimov, Ulrike Kraft, Weimin Zhang, Maryam Alsufyani, Iain McCulloch, M. Roisin Owens, Per M. Claesson, Tuomas P. J. Knowles, Henning Sirringhaus

Small Methods, 7, 11, 2300476

Link zur Publikation

Zusammenfassung:

Organic semiconductors are a family of pi-conjugated compounds used in many applications, such as displays, bioelectronics, and thermoelectrics. However, their susceptibility to processing-induced contamination is not well understood. Here, it is shown that many organic electronic devices reported so far may have been unintentionally contaminated, thus affecting their performance, water uptake, and thin film properties. Nuclear magnetic resonance spectroscopy is used to detect and quantify contaminants originating from the glovebox atmosphere and common laboratory consumables used during device fabrication. Importantly, this in-depth understanding of the sources of contamination allows the establishment of clean fabrication protocols, and the fabrication of organic field effect transistors (OFETs) with improved performance and stability. This study highlights the role of unintentional contaminants in organic electronic devices, and demonstrates that certain stringent processing conditions need to be met to avoid scientific misinterpretation, ensure device reproducibility, and facilitate performance stability. The experimental procedures and conditions used herein are typical of those used by many groups in the field of solution-processed organic semiconductors. Therefore, the insights gained into the effects of contamination are likely to be broadly applicable to studies, not just of OFETs, but also of other devices based on these materials.

Platform-agnostic electrochemical sensing app and companion potentiostat

Wissenschaftlicher Artikel ArODES

Vijayalaxmi Manoharan, Rui Rodrigues, Sara Sadati, Marcus B. Swann, Neville Freeman, Bowen Du, Ender Yildirim, Ugur Tamer, Theodoros N. Arvanitis, Dmitry Isakov, Ali Asadipour, Jérôme Charmet

The Analyst,

Link zur Publikation

Zusammenfassung:

Electrochemical sensing is ubiquitous in a number of fields ranging from biosensing, to environmental monitoring through to food safety and battery or corrosion characterisation. Whereas conventional potentiostats are ideal to develop assays in laboratory settings, they are in general, not well-suited for field work due to their size and power requirements. To address this need, a number of portable battery-operated potentiostats have been proposed over the years. However, most open source solutions do not take full advantage of integrated circuit (IC) potentiostats, a rapidly evolving field. This is partly due to the constraining requirements inherent to the development of dedicated interfaces, such as apps, to address and control a set of common electrochemical sensing parameters. Here we propose the PocketEC, a universal app that has all the functionalities to interface with potentiostat ICs through a user defined property file. The versatility of PocketEC, developed with an assay developer mindset, was demonstrated by interfacing it, via Bluetooth, to the ADuCM355 evaluation board, the open-source DStat potentiostat and the Voyager board, a custom-built, small footprint potentiostat based around the LMP91000 chip. The Voyager board is presented here for the first time. Data obtained using a standard redox probe, Ferrocene Carboxylic Acid (FCA) and a silver ion assay using anodic stripping multi-step amperometry were in good agreement with analogous measurements using a bench top potentiostat. Combined with its Voyager board companion, the PocketEC app can be used directly for a number of wearable or portable electrochemical sensing applications. Importantly, the versatility of the app makes it a candidate of choice for the development of future portable potentiostats. Finally, the app is available to download on the Google Play store and the source codes and design files for the PocketEC app and the Voyager board are shared via Creative Commons license (CC BY-NC 3.0) to promote the development of novel portable or wearable applications based on electrochemical sensing.

Rapid tooling :

Wissenschaftlicher Artikel ArODES

investigation of soft-tooled micro-injection moulding process characteristics using in-line measurements and surface metrology

Mert Gülçür, Kevin Couling, Vannessa Goodship, Jérôme Charmet, Gregory J. Gibbons

Rapid Prototyping Journal, 29, 7, 1420-1437

Link zur Publikation

Zusammenfassung:

The purpose of this study is to demonstrate and characterise a soft-tooled micro-injection moulding process through in-line measurements and surface metrology using a data-intensive approach. A soft tool for a demonstrator product that mimics the main features of miniature components in medical devices and microsystem components has been designed and fabricated using material jetting technique. The soft tool was then integrated into a mould assembly on the micro-injection moulding machine, and mouldings were made. Sensor and data acquisition devices including thermal imaging and injection pressure sensing have been set up to collect data for each of the prototypes. Off-line dimensional characterisation of the parts and the soft tool have also been carried out to quantify the prototype quality and dimensional changes on the soft tool after the manufacturing cycles. The data collection and analysis methods presented here enable the evaluation of the quality of the moulded parts in real-time from in-line measurements. Importantly, it is demonstrated that soft-tool surface temperature difference values can be used as reliable indicators for moulding quality. Reduction in the total volume of the soft-tool moulding cavity was detected and quantified up to 100 cycles. Data collected from in-line monitoring was also used for filling assessment of the soft-tool moulding cavity, providing about 90% accuracy in filling prediction with relatively modest sensors and monitoring technologies. This work presents a data-intensive approach for the characterisation of soft-tooled micro-injection moulding processes for the first time. The overall results of this study show that the product-focussed data-rich approach presented here proved to be an essential and useful way of exploiting additive manufacturing technologies for soft-tooled rapid prototyping and new product introduction.

X-ray computed tomography for predictive quality assessment, 3D visualisation of micro-injection mouldings and soft-tool deformation

Wissenschaftlicher Artikel ArODES

Mert Gülçür, Paul Wilson, Michael Donnelly, Kevin Couling, Vanessa Goodship, Jérôme Charmet, Mark A. Williams, Gregory Gibbons

Materials Design, 2023, vol. 227, article no. 111741

Link zur Publikation

Zusammenfassung:

This work presents X-ray computed tomography (XCT) as a dimensional quality assurance technique for micro-injection moulded polymeric test objects for the establishment of predictive quality models and quantifying soft-tool deformation. The results are compared against an industry standard laser-scanning-confocal microscope (LSCM) for the evaluation of XCT’s capability. The work demonstrates; (i) the exploitation of a XCT equipment for dimensional characterisation of micro-injection moulded products made out of polymers with adequate acquisition times, (ii) that acquired XCT data from the 3D visualisation of the micromouldings perform on par with a laser-scanning-confocal microscope in a quality prediction model, (iii) that the deformation occurring in an additively manufactured soft-tool can be quantified using XCT. The technique was particularly superior in volumetric data acquisition compared to LSCM in the filling prediction of the micromouldings. Better accuracy and repeatability in predicting the quality of the mouldings up to 92% achieved with XCT, in conjunction with an in-line collected soft-tool surface temperature data as an indirect quality assurance method. Given the capability of the XCT for the 3D data acquisition of polymeric miniature components, the approach described here has great potential in high-value micro-manufacturing process quality modelling for in-line quality assessment of miniature and added value products in data-rich contexts. Rendered 3D animation of the X-ray CT data: https://youtu.be/KwZty_yoDfs.

Flow rate-independent multiscale liquid biopsy for precision oncology

Wissenschaftlicher Artikel ArODES

Jie Wang, Robert Dallmann, Renquan Lu, Jing Yan, Jérôme Charmet

ACS Sensors, 8, 3, 1200-1210

Link zur Publikation

Zusammenfassung:

Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management but typically suffer from low throughput, relative complexity, and postprocessing limitations. Here, we address these issues simultaneously by decoupling and independently optimizing the nano-, micro-, and macro-scales of an enrichment device that is simple to fabricate and operate. Unlike other affinity-based devices, our scalable mesh approach enables optimum capture conditions at any flow rate, as demonstrated with constant capture efficiencies, above 75% between 50 and 200 μL min–1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. We demonstrate its postprocessing capacity with the identification of potential responders to immune checkpoint inhibition (ICI) therapy and the detection of HER2 positive breast cancer. The results compare well with other assays, including clinical standards. This suggests that our approach, which overcomes major limitations associated with affinity-based liquid biopsies, could help improve cancer management.

2022

Multifunctional composite hydrogels for bacterial capture, growth/elimination, and sensing applications

Wissenschaftlicher Artikel ArODES

Andrea Dsouza, Chrystala Constatinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet, Rachel A. Hand

ACS Applied Materials Interfaces, 2022, 14, 42, 47323-47344

Link zur Publikation

Zusammenfassung:

Hydrogels are crosslinked networks of hydrophilic polymer chains with a three-dimensional structure. Owing to their unique features, the application of hydrogels for bacterial/antibacterial studies and bacterial infection management has grown in importance in recent years. This trend is likely to continue due to the rise in bacterial infections and antimicrobial resistance. By exploiting their physiochemical characteristics and inherent nature, hydrogels have been developed to achieve bacterial capture and detection, bacterial growth or elimination, antibiotic delivery, or bacterial sensing. Traditionally, the development of hydrogels for bacterial/antibacterial studies have focused on achieving a single function such as antibiotic delivery, antibacterial activity, bacterial growth, or bacterial detection. However, recent studies demonstrate the fabrication of hydrogels that exhibit multifunctionality in the form of multifunctional hydrogels, where a single hydrogel is capable of performing more than one bacterial/antibacterial function or composite hydrogels consisting of a number of single functionalized hydrogels, which exhibit bacterial/antibacterial function synergistically. In this review, we first highlight the hydrogel features critical for bacterial studies and infection management. Then, we specifically address unique hydrogel properties, their surface/network functionalization, and mode of action for bacterial capture, adhesion/growth, antibacterial activity, and bacterial sensing respectively. Finally, we provide insights into different strategies for developing multifunctional hydrogels and how such systems can help tackle, manage, and understand bacterial infections and antimicrobial resistance. We also note that the strategies highlighted in this review can be adapted to other cell types and are therefore likely to find applications beyond the field of microbiology.

Flow rate independent multiscale liquid biopsy for precision oncology

Wissenschaftlicher Artikel ArODES

Jing Yan, Jie Wang, Robert Dallmann, Renquan Lu, Jérôme Charmet

arXiv.org,

Link zur Publikation

Zusammenfassung:

Immunoaffinity-based liquid biopsies of circulating tumour cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues simultaneously by decoupling and independently optimising the nano-, micro- and macro-scales of a CTC enrichment device that is both simple to fabricate and operate. At its core is a scalable macroscale mesh with optimised micropores, nanofunctionalised with antibodies against cell surface proteins. Unlike other affinity-based liquid biopsies, optimum capture can be achieved independently of the flow rate, as demonstrated with constant capture efficiencies, above 75%, between 50–200 µL min-1. The device achieved 96% sensitivity and 100% specificity when used to detect CTCs in the blood of 79 cancer patients and 20 healthy controls. To demonstrate its post-processing capabilities, we used immunofluorescence labelling to identify PD-L1+ CTCs in 36% of patients (n=33) as potential responders to immune checkpoint inhibition therapy. Finally, our device achieved an 80% positive match in the identification of HER2+ breast cancer (n=26) compared to clinical standard FISH on solid biopsy. The results suggest that our approach, which overcomes major limitations previously associated with affinity-based liquid biopsies, could provide a versatile tool to improve cancer management.

P10 rapid capture of uropathogenic bacteria and on-chip determination of antimicrobial resistance

Professioneller Artikel ArODES

Andrea Dsouza, Rachel Hand, Chrystala Constantinidou, Theodoros N. Arvanitis, David M. Haddleton, Jérôme Charmet

JAC-Antimicrobial Resistance, 2022, vol. 4, suppl. no. 1

Link zur Publikation

Publications (sélection parmi >50 publications, >1200 citations, h-index 18)

Wissenschaftlicher Artikel

Charmet Jérôme

Selection, 2022

Zusammenfassung:

Liste complète ici

T. Kartanas et al., Analytical Chemistry 93, 2848, 2021
T. Kartanas et al., Applied Physics Letters, 116, 153702, 2020.
J. Charmet et al., Micromachines, 11, 135, 2020.
C. Bortolini, et al. Lab on a Chip, 19, 50, 2019.
R.S. Ruggeri, et al. Nature Communication 9, 3890, 2018
W.E. Arter, et al. Analytical Chemistry. 90, 10302, 2018.
J. Charmet, P. Arosio, T. P. J. Knowles, Journal of Molecular Biology. 430, 565, 2018.
T. Kartanas et al., Analytical Chemistry. 89, 11929, 2017.
M. Nikolka, et al., Nature Materials. 16, 356, 2017.
P. K. Challa, et al., Biomicrofluidics 11, 0141131, 2017.
J. Charmet, et al., Physical Review Applied. 5, 064016, 2016.
A. Prasad, J. Charmet, A. A. Seshia, Sensors and Actuators A Physical. 238, 207, 2016.
J. Charmet, et al., Applied Physics Letters. 105, 013502, 2014.

2022

Flow rate effect on THz-TDS of thin-film fluid in microfluidic device

Konferenz ArODES

Xuefei Ding, Jérôme Charmet, Emma Pickwell-McPherson

Proceedings of the 2022 47th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz), 28 August - 2 September 2022, Delft, Netherlands

Link zur Konferenz

Zusammenfassung:

Here we present a compact microfluidic device for thin-film water fluid measurements in reflection THz-TDS. We discuss the variables that need to be considered when utilizing this device in THz-TDS, and we find that different flow rates can cause subtle changes in the THz response.

Errungenschaften

PEOPLE@HES-SO
Verzeichnis der Mitarbeitenden und Kompetenzen

Charmet Jérôme

Professeur-e HEs associé-e B

Hauptkompetenzen

Biosensors

Sample Processing

Microfluidics

Advanced Manufacturing

Functional materials

Professeur-e HEs associé-e B

PEOPLE@HES-SO Verzeichnis der Mitarbeitenden und Kompetenzen

Charmet Jérôme

Professeur-e HEs associé-e B

Hauptkompetenzen

Biosensors

Sample Processing

Microfluidics

Advanced Manufacturing

Functional materials

Professeur-e HEs associé-e B

PEOPLE@HES-SO
Verzeichnis der Mitarbeitenden und Kompetenzen